藏红花花被、柱头和叶片氢乙醇提取物对四氯化碳致大鼠急性肝损伤的保护作用

IF 0.3 Q4 PHYSIOLOGY

Physiology and Pharmacology Pub Date : 2021-07-10 DOI:10.32598/PPJ.25.2.30

S. Ouahhoud, Ilham Touiss, Amine Khoulati, I. Lahmass, Samira Mamri, M. Meziane, S. Elassri, N. Bencheikh, Redouane Benabbas, A. Asehraou, M. Choukri, E. Saalaoui

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引用次数: 5

摘要

摘要:本研究探讨了藏红花柱头、花被片和叶片对四氯化碳(CCL4)致大鼠肝损伤的保护作用。方法:以藏红花(柱头、花被、叶片)的乙醇提取物灌胃，每天灌胃14 d。本研究选取30只雄性大鼠，分为5组:1 .正常大鼠灌胃蒸馏水;2:用蒸馏水灌胃并注射CCL4;3:大鼠给予柱头提取物并注射CCL4;4:大鼠灌胃花被提取物并注射CCL4;5:给大鼠叶提取物和注射CCL4。测定体重和相对肝脏重量。测定血浆丙氨酸转氨酶(ALT)、天冬氨酸转氨酶(AST)、碱性磷酸酶(ALP)、乳酸脱氢酶(LDH)、总胆固醇、甘油三酯、直接和总胆红素、总蛋白、白蛋白、尿素和肌酐。测定肝脏匀浆中丙二醛(MDA)含量。结果:实验数据表明，柱头和花被提取物能显著防止大鼠体重减轻，改善肝脏相对重量。它们对ALT、AST、直接胆红素、总胆红素、LDH、ALP、肌酐和MDA的升高有显著的保护作用。此外，与CCL4对照组相比，它们显著提高了总蛋白和白蛋白。此外，叶片显著降低了ALT、AST、总胆红素、LDH和MDA。结论:红花被片、柱头和叶提取物对CCL4诱导的大鼠肝损伤具有保护作用。

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Hepatoprotective effects of hydroethanolic extracts of Crocus sativus tepals, stigmas and leaves on carbon tetrachloride induced acute liver injury in rats

Introduction: The present study investigated the hepatoprotective effects of stigmas, tepals and leaves of Crocus sativus on carbon tetrachloride (CCL4) induced liver injury in rats. Methods: Hydroethanolic extracts of Crocus sativus (stigmas, tepals and leaves) were administrated daily for 14 days by oral gavage. In the present study, 30 male rats divided into five groups were treated as 1: normal rats gavaged with distilled water; 2: intoxicated rats gavaged with distilled water and injected with CCL4; 3: rats treated with stigmas extract and injected with CCL4; 4: rats treated with tepal extract and injected with CCL4; 5: rats treated with leaf extract and injected with CCL4. Bodyweight and the relative liver weight were determined. Alanine aminotransferase (ALT), aspartate aminotransferase (AST), alkaline phosphatase (ALP), lactate dehydrogenase (LDH), total cholesterol, triglycerides, bilirubin direct and total, total protein, albumin, urea and creatinine measured in plasma. Malondialdehyde (MDA) was quantified in liver homogenate. Results: The experimental data showed that the stigmas and tepals extracts significantly prevented weight body loss and improved the relative liver weight. They significantly protected against elevation of ALT, AST, direct bilirubin, total bilirubin, LDH, ALP, creatinine and MDA. Also, they enhanced significantly total proteins and albumin compared to the CCL4 control group. Moreover, leaves reduced ALT, AST, total bilirubin, LDH and MDA significantly. Conclusion: In conclusion, these results suggest that tepals, stigmas, and leaves extracts of Crocus sativus have hepatoprotective effects on CCL4 induced liver injury in rats.

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Physiology and Pharmacology PHYSIOLOGY-

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期刊介绍： Physiology and Pharmacology is the official English publication of the Iranian Society of Physiology and Pharmacology. The journal publishes Review articles, Full-length original articles, Letter to editor and Short communications in physiology, pharmacology and related subjects. The aim of this journal is to provide a medium of scientific communication for investigators in the field of Physiology and Pharmacology. The editors will welcome original basic and applied research articles from Physiologists and Pharmacologists. Articles should be in English language. The papers submitted to this journal must not be Published or under consideration for publication elsewhere. Physiology and Pharmacology is an open access journal which means that all contents is freely available without charge to the user or his/her institution. Users are allowed to read, download, copy, distribute, print, search or link to the full text of the articles in this journal without asking prior permission from the publisher or the author.