木耳全株提取物体外抗氧化和抗癌活性的初步筛选

Q3 Pharmacology, Toxicology and Pharmaceutics

Biomedical and Pharmacology Journal Pub Date : 2023-06-30 DOI:10.13005/bpj/2677

Nagaraju Kancherla, Anusha Dhakshinmoothi, K. Chitra, J. Palla, Ravi Babu Komaram

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引用次数: 0

摘要

肿瘤的高增殖性导致健康细胞的自然稳态被破坏，从而刺激多种基因，这些基因密集地参与细胞周期、存活、血管生成和转移。本研究主要研究了药用植物荆芥(Cayratia auriculata)全株提取物的体外抗氧化和细胞毒活性。采用索氏提取法，以正己烷<氯仿>乙酸乙酯<甲醇的极性顺序进行提取。采用标准的生化方法对各组分进行了植物成分的筛选。通过测定总酚含量(TFC)、铁还原抗氧化能力(FRAP)、一氧化氮清除能力、2,2 -二苯基-1-苦味酰肼(DPPH)和总抗氧化能力(TAC)等方法对其抗氧化能力进行定量研究。采用(3-(4,5 -二甲基噻唑-2基)- 2,5 -二苯基溴化四氮唑)MTT法评价了A549肺癌细胞株的体外细胞毒活性。植物化学分析表明，4个馏分均存在不同程度的次生生物活性代谢物，但甲醇馏分具有丰富的植物成分。甲醇馏分表现出较好的总酚含量、较强的抗氧化潜力、较强的FRAP、DPPH、一氧化氮清除活性和总抗氧化活性。MTT实验显示，木耳甲醇组分对肺癌细胞株A549的细胞毒作用最强，其抑制浓度(IC50)为115.14µg/ml。本研究结果表明，木耳不同部位的植物化学物质、总酚含量和抗氧化活性存在不同程度的差异。甲醇组分显示出丰富的植物化学物质，总酚含量，有效的抗氧化和抗癌特性(体外)。

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Preliminary Screening for In-Vitro Antioxidant and Anticancer Potentials in Whole Plant Fractions of Cayratia Auriculata (Vitaceae)

Failure of natural homeostasis of healthy cell due to hyperproliferative nature of cancer, resulting in stimulation of various genes which are intensively participated in cell cycle, survival, angiogenesis and metastasis. Our study focused on the in-vitro antioxidant and cytotoxic potentials of the whole plant fractions of Cayratia auriculata, a medicinal plant belongs to Vitaceae family. The whole plant material was shade dried and powdered, fractions were prepared by using soxhlet extraction technique with the ascending order of polarity such as hexane < chloroform, < ethyl acetate < methanol. Screening for phytoconstituents in fractions was carried with standard biochemical instigations. Quantitative investigation was done by using different assays such as total phenolic content (TFC), ferric reducing antioxidant power (FRAP) assay, nitric oxide scavenging activity, 2,2 -Diphenyl-1-picrylhydrazyl (DPPH) assay and total antioxidant activity (TAC) to reveal antioxidant capacity. In-vitro cytotoxicity activity on A549 lung cancer cell line was evaluated by (3-(4, 5-dimethyl thiazole-2yl)-2, 5-diphenyl tetrazolium bromide) MTT assay. Phytochemical analysis of all four fractions showed the existence of varying degree of secondary bioactive metabolites but methanol fraction exhibited richness in phytoconstituents. Methanol fraction revealed good total phenolic content, potent antioxidant potential in FRAP, DPPH, Nitric oxide scavenging activity and total antioxidant activity in contrast to other tested fractions. MTT assay revealed that methanol fraction C. auriculata has strongest cytotoxic effect towards (A549) lung cancer cell line with an inhibitory concentration (IC50) value of 115.14 µg/ml. The results of present study indicate that different fractions of C. auriculata showed the existence of varying degree of phytochemicals, total phenolic content and dose dependent antioxidant activity. Methanolic fraction revealed richness in phytochemicals, total phenolic content, potent antioxidant, and anticancer property (in- vitro).

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来源期刊

Biomedical and Pharmacology Journal Pharmacology, Toxicology and Pharmaceutics-Pharmacology

CiteScore

1.20

自引率

0.00%

发文量

189

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