Effect of soya-milk based extender on the physico-morphological parameters of Murrah bull semen during cryopreservation

Present investigation was undertaken on three buffalo bulls (Murrah) to study the effect of cryopreservation using Soya-milk extender. A total of 18 collections from three bulls (six collections per bull) were utilized for the study. After initial evaluation, each semen sample was diluted in two different extenders viz. Tris egg yolk extender which acted as control and Soya-milk extender as experimental. Various sperm parameters were studied at equilibration and post-thaw stages. There was no significant difference for percent head abnormalities, percent midpiece abnormalities and acrosome integrity of spermatozoa among extenders at different stages of freezing. The progressive motility and live sperm percentage was significantly higher for Tris egg yolk extender at dilution, equilibration and postthaw stages, but the values for Soya-Milk were also within acceptable limits at all stages of freezing as compared to Tris egg yolk extender. Furthermore the percent tail abnormalities and total sperm abnormalities were significantly higher for soya-milk extender at post thaw. In conclusion the experimental extender could be used as an alternate plant based extender in the cryopreservation of bubaline semen.