结晶紫-激素法测定大鼠发情周期的简便快速方法

N. Begum, K. Manipriya, Rahathunnisa Begum, B. Veeresh
{"title":"结晶紫-激素法测定大鼠发情周期的简便快速方法","authors":"N. Begum, K. Manipriya, Rahathunnisa Begum, B. Veeresh","doi":"10.21477/IJAPSR.5.4.1","DOIUrl":null,"url":null,"abstract":"Rat estrous cycle determination or vaginal smear staining is paramount for studies related to endocrinology\nand reproduction; in the present study; we have reported a simple and rapid method for estrous cycle determination\nin rats using crystal violet. With this technique, the identification of stages can be done even on the\nnext day. Hormonal variation in blood and histomorphological changes in ovaries at different stages of the\nestrous cycle were studied in female Wistar rats, which can be used to determine the hormone levels in works\nrelated to hormonal drugs, further ovarian morphology can be used to study changes in ovaries during the\nestrous cycle. This study aims to report a rapid and simple method for vaginal cytology using crystal violet\nand to report normal hormonal levels and histomorphology of ovaries in various phases of the estrous cycle.","PeriodicalId":13749,"journal":{"name":"INTERNATIONAL JOURNAL OF APPLIED PHARMACEUTICAL SCIENCES AND RESEARCH","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"2020-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"1","resultStr":"{\"title\":\"Simple and Rapid Method for Rat Estrous Cycle Identification Using Crystal Violet- Hormonal Consideration\",\"authors\":\"N. Begum, K. Manipriya, Rahathunnisa Begum, B. Veeresh\",\"doi\":\"10.21477/IJAPSR.5.4.1\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Rat estrous cycle determination or vaginal smear staining is paramount for studies related to endocrinology\\nand reproduction; in the present study; we have reported a simple and rapid method for estrous cycle determination\\nin rats using crystal violet. With this technique, the identification of stages can be done even on the\\nnext day. Hormonal variation in blood and histomorphological changes in ovaries at different stages of the\\nestrous cycle were studied in female Wistar rats, which can be used to determine the hormone levels in works\\nrelated to hormonal drugs, further ovarian morphology can be used to study changes in ovaries during the\\nestrous cycle. This study aims to report a rapid and simple method for vaginal cytology using crystal violet\\nand to report normal hormonal levels and histomorphology of ovaries in various phases of the estrous cycle.\",\"PeriodicalId\":13749,\"journal\":{\"name\":\"INTERNATIONAL JOURNAL OF APPLIED PHARMACEUTICAL SCIENCES AND RESEARCH\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2020-06-30\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"1\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"INTERNATIONAL JOURNAL OF APPLIED PHARMACEUTICAL SCIENCES AND RESEARCH\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.21477/IJAPSR.5.4.1\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"INTERNATIONAL JOURNAL OF APPLIED PHARMACEUTICAL SCIENCES AND RESEARCH","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.21477/IJAPSR.5.4.1","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 1

摘要

大鼠发情周期测定或阴道涂片染色在内分泌学和生殖学研究中至关重要;在目前的研究中;本文报道了一种用结晶紫测定大鼠发情周期的简便、快速方法。使用这种技术,甚至可以在第二天进行阶段识别。研究了雌性Wistar大鼠在不同发情周期的血液激素变化和卵巢组织形态学变化,可用于确定激素药物相关工作中的激素水平,进一步利用卵巢形态学研究发情周期中卵巢的变化。本研究旨在报告一种快速、简便的阴道细胞学检测方法,并报告卵巢在发情周期各阶段的正常激素水平和组织形态。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Simple and Rapid Method for Rat Estrous Cycle Identification Using Crystal Violet- Hormonal Consideration
Rat estrous cycle determination or vaginal smear staining is paramount for studies related to endocrinology and reproduction; in the present study; we have reported a simple and rapid method for estrous cycle determination in rats using crystal violet. With this technique, the identification of stages can be done even on the next day. Hormonal variation in blood and histomorphological changes in ovaries at different stages of the estrous cycle were studied in female Wistar rats, which can be used to determine the hormone levels in works related to hormonal drugs, further ovarian morphology can be used to study changes in ovaries during the estrous cycle. This study aims to report a rapid and simple method for vaginal cytology using crystal violet and to report normal hormonal levels and histomorphology of ovaries in various phases of the estrous cycle.
求助全文
通过发布文献求助,成功后即可免费获取论文全文。 去求助
来源期刊
自引率
0.00%
发文量
0
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信