{"title":"双孢蘑菇子实体中镉结合成分的分离与部分纯化","authors":"J. Esser, H. Brunnert","doi":"10.1016/0143-1471(86)90074-7","DOIUrl":null,"url":null,"abstract":"<div><p>Cadmium speciation in the commercially important species <em>Agaricus bisporus</em> was investigated by comparing the binding of Cd in the cytosolic fractions obtained from fruiting bodies containing < 1 and 70 mg Cd kg<sup>−1</sup> (dry weight). Three Cd-binding complexes which are constitutive were isolated and partially characterised. Gel filtration, ion exchange and affinity chromatography were used in the purification procedure. Metallothionein-like components were not detected.</p></div>","PeriodicalId":100483,"journal":{"name":"Environmental Pollution Series A, Ecological and Biological","volume":"41 3","pages":"Pages 263-275"},"PeriodicalIF":0.0000,"publicationDate":"1986-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0143-1471(86)90074-7","citationCount":"21","resultStr":"{\"title\":\"Isolation and partial purification of cadmium-binding components from fruiting bodies of Agaricus bisporus\",\"authors\":\"J. Esser, H. Brunnert\",\"doi\":\"10.1016/0143-1471(86)90074-7\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>Cadmium speciation in the commercially important species <em>Agaricus bisporus</em> was investigated by comparing the binding of Cd in the cytosolic fractions obtained from fruiting bodies containing < 1 and 70 mg Cd kg<sup>−1</sup> (dry weight). Three Cd-binding complexes which are constitutive were isolated and partially characterised. Gel filtration, ion exchange and affinity chromatography were used in the purification procedure. Metallothionein-like components were not detected.</p></div>\",\"PeriodicalId\":100483,\"journal\":{\"name\":\"Environmental Pollution Series A, Ecological and Biological\",\"volume\":\"41 3\",\"pages\":\"Pages 263-275\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"1986-01-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://sci-hub-pdf.com/10.1016/0143-1471(86)90074-7\",\"citationCount\":\"21\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Environmental Pollution Series A, Ecological and Biological\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/0143147186900747\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Environmental Pollution Series A, Ecological and Biological","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/0143147186900747","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 21
摘要
通过比较双孢蘑菇(Agaricus bisporus)子实体中镉的结合，研究了具有重要商业价值的双孢蘑菇(Agaricus bisporus)中镉的形态。1和70 mg Cd kg - 1(干重)。分离了三个本构性的cd结合配合物，并对其进行了部分表征。采用凝胶过滤、离子交换、亲和层析等方法进行纯化。未检出金属硫蛋白样成分。
Isolation and partial purification of cadmium-binding components from fruiting bodies of Agaricus bisporus
Cadmium speciation in the commercially important species Agaricus bisporus was investigated by comparing the binding of Cd in the cytosolic fractions obtained from fruiting bodies containing < 1 and 70 mg Cd kg−1 (dry weight). Three Cd-binding complexes which are constitutive were isolated and partially characterised. Gel filtration, ion exchange and affinity chromatography were used in the purification procedure. Metallothionein-like components were not detected.
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