UV-B辐射下TUB-GFP和MBD-GFP拟南芥微管的比较效应

4区农林科学

Tarim Bilimleri Dergisi-journal of Agricultural Sciences Pub Date : 2017-09-06 DOI:10.15832/TBD.29540

D. Ma, Huize Chen, R. Han

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引用次数: 1

摘要

本研究以拟南芥(Arabidopsis thaliana)为材料，利用共聚焦激光扫描显微镜，比较了UV-B辐射对拟南芥株高、初生根长以及微管动力学的影响。结果表明，与野生型相比，转基因株系对UV-B辐射具有相同的损伤效应，且转基因株系对UV-B更为敏感。MBD-GFP拟南芥能更好地适应UV-B处理的变化。因此，这些结果表明，UV-B可以抑制转基因拟南芥的生长发育，其抑制作用可能与微管的变化有关。

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The comparative effects of microtubules in TUB-GFP and MBD-GFP Arabidopsis thaliana exposed to UV-B Radiation

In the present study, we used the TUB-GFP and MBD-GFP of the Arabidopsis thaliana , compared with effects of the plant height and primary root length, and changes of microtubules dynamics by confocal laser scanning microscope after UV-B radiation. It was shown that UV-B radiation had the same damaged effects on the transgenic lines, compared with wild type, and the transgenic lines were more sensitive for UV-B. MBD-GFP Arabidopsis thaliana could be better adapted to the changes with UV-B treatment. Therefore, these results indicated that UV-B could inhibit the growth and the development of transgenic Arabidopsis thaliana , and the inhibition effects might result from changes in microtubules.

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来源期刊

Tarim Bilimleri Dergisi-journal of Agricultural Sciences 农林科学-农业综合

CiteScore

1.40

自引率

0.00%

发文量

期刊介绍： Journal of Agricultural Sciences (JAS) is an international, double-blind peer-reviewed, open-access journal, published by the Faculty of Agriculture, Ankara University. The journal invites original research papers containing new insight into any aspect of Agricultural Sciences that are not published or not being considered for publication elsewhere. Preliminary, confirmatory or inconclusive research, review articles, case and local studies and works presenting taxonomy will not be published.