Rustem S. Medora , Galen P. Mell , David E. Bilderback
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Effect of Various Media on Growth and Protease Production in Carica papaya L. Callus Cultures
Stock callus cultures of Carica papaya L. maintained in modified White's medium were grown in twenty media types. Growth, protease activity and total protein in extratives is reported. The basic Wood and Braun medium produced good growth and high protease activity. Reduction of macro- and/or microelements in Murashige and Skoog basic medium increased protein content and enzyme activity of the callus. In most cases, peptone was found to significantly increase growth, protease activity and protein content of callus cultures.
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