小黑麦双单倍体系系内多态性及纯合率的微卫星标记分析

В. Лагуновская, А. А. Буракова, Н. Буштевич, И. Сакович, В. А. Лемеш, И. Гриб, A. V. Lagunovskaya, Aryna А. Buracova, V. N. Bushtevich, V. Sakovich, Valiantsina A. Lemesh, Academician Stanislau I. Gryb
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引用次数: 1

摘要

以春型和冬型杂交品种为基础,对花药培养法获得的六倍体小黑麦双单倍体系的多态性进行了评价。利用A- (Xgwm186、Xgwm291、Xgwm595)和B- (Xgwm371、Xgwm540、Xgwm554、Xgwm234)染色体位点上的7个SSR标记,对38个六倍体小黑麦双倍单倍体系的多态性进行了研究。除Xgwm554位点外,其余6个微卫星位点均存在多态性,该位点在双单倍体系中不存在多态性。多态性最高的位点为Xgwm186、Xgwm291和Xgwm595。聚类分析表明,所有研究品系可分为3个主要类群。品系的起源对群体分布没有影响。这证实了离体培养体细胞无性系变异的影响。鉴定出8个双单倍体系,其中一个微卫星位点为杂合性。利用SSR标记对体外诱导雄激素发生法获得的小黑麦双单倍体系进行系间多态性和纯合子率评价的可能性。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Analysis of the intralinear polymorphism and the homozygosity rate of triticale doubled haploid lines by microsatellite markers
We evaluated the rate of polymorphism of doubled haploid lines of hexaploid triticale obtained by the method of anther culture based on hybrids of spring and winter types. Using 7 SSR markers for the loci on the chromosomes A- (Xgwm186, Xgwm291, Xgwm595) and B- (Xgwm371, Xgwm540, Xgwm554, Xgwm234), polymorphism of 38 doubled haploid lines of hexaploid triticale was studied. Interlinear polymorphism along six microsatellite loci except the Xgwm554 locus, which is not polymorphic in the studied doubled haploid lines, was revealed. The highest polymorphism was observed for the Xgwm186, Xgwm291 and Xgwm595 loci. The cluster analysis showed that all studied lines were divided into three main groups. The origin of the lines did not affect the distribution in groups. This confirms the influence of in vitro culture somaclonal variation. Eight lines of doubled haploids, which are heterozygous for one of the studied microsatellite loci, were identified. We showed the possibility of using SSR markers to assess interlinear polymorphism and the homozygosity rate in the triticale doubled haploid lines obtained by the method of induced androgenesis in vitro.
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