枸橼酸磷酸葡萄糖腺嘌呤-1(CPDA-1)血中血浆血红蛋白浓度的变化

E. Eze, S. Christian, Ransom Baribefii Jacob, Z. Jeremiah, Iminabo Diada Walter Chuku
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引用次数: 2

摘要

目的:本研究评估CPDA-1贮存血中血浆血红蛋白浓度水平,以确定35天贮存期内溶血的程度。研究设计:这是一项观察性和比较病例对照研究。研究地点和时间:研究使用居住在哈科特港的健康男性供体。分析于2017年2月1日至3月8日在尼日利亚哈科特港河流州立大学教学医院血库(原Braithwaite纪念专科医院(BMSH))进行。方法:对健康状况良好、年龄在18 ~ 52岁、血红蛋白水平在13.5 g/dl ~ 16 g/dl、体重在55 kg ~ 75 kg、体温在37.0 ~ 37.50℃/ 99.50F之间的男性献血者采集输血血液,装入含有抗凝剂CPDA-1的塑料袋中,在严格的无菌条件下处理,防止细菌污染。将血液保存在恒温于+2至+60℃的血库冰箱中,每隔一段时间进行适当的检查,检查血液的颜色、浑浊度、溶血情况和凝块形成情况。在不同的采集间隔天从血袋中无菌采集2毫升样品,使用HemoCue光度计进行分析。结果:血浆血红蛋白在第1、5、10天无显著变化,而溶血在第15、20、25、30、35天显著增加(p = 0.000),血浆血红蛋白在第15 ~ 35天显著增加(p<0.05)。结论:因此值得注意的是,使用CPDA-1并不能完全阻止红细胞发生的变化,因为在储存的血液中发生了几种变化,统称为“储存病变”。因此,建议在储存后14天内输血,以避免输血的血液制品对受者已经失去了大部分的益处,并且在可能的情况下,全血应在储存前进行处理并分离成分,以减少不能存活的红细胞的水平。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Changes in Plasma Haemoglobin Concentration in Citrate Phosphate Dextrose Adenine-1(CPDA-1) Stored Blood
Aim: This study assessed the level of plasma haemoglobin concentration in CPDA-1 stored blood with a view to determine the extent of haemolysis during the 35 days storage period. Study Design:  This is an observational and comparative case-control study. Place and Duration of Study: The study was conducted using healthy male donors residing in Port Harcourt. Analysis was carried out at the Blood Bank of Rivers State University Teaching Hospital, formerly Braithwaite Memorial Specialist Hospital (BMSH), Port Harcourt, Nigeria, from February 1st to March 8th, 2017. Methodology: Blood for transfusion was collected from prospective male blood donor found to be in good health, aged between 18 and 52 years, with haemoglobin level within the range of 13.5 g/dl – 16 g/dl, body weight within 55 kg – 75 kg, and body temperature within 37.0 to 37.50C / 99.50F, into plastic bags containing anticoagulant CPDA-1, and handled under strict sterile condition to prevent bacterial contamination. The blood was stored in a blood bank refrigerator with a constant temperature of +2 to +60C under proper inspection at intervals for colour, turbidity, haemolysis and clot formation. Two milliliters of the sample was collected aseptically at different interval days of collection from the blood bag and analyzed using the HemoCue photometer. Results: Results showed no significant changes in plasma haemoglogin from day 1, 5, and 10, while significant increase in haemolysis occurred from day 15, 20, 25, 30, and 35 (p = 0.000), a significant increase (p<0.05) in plasma haemoglobin was observed from day 15 to day 35 of storage. Conclusion: It is pertinent therefore to note that the use of CPDA-1 does not completely stop the changes that occur in RBC as there are several changes occurring in stored blood collectively called “storage lesions”. Therefore, it is advisable that blood should be transfused within 14 days of storage to avoid transfusion of blood products that has lost most of its benefits to recipients, and where possible whole blood should be processed and components separated before storage to reduce the level of non-viable red blood cells.
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