不同剂量鲶鱼脑垂体提取物对恒潜伏期非洲鲶鱼卵和孵化量的影响

Sebastian S Moshaand, F. T. Mlingi
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引用次数: 1

摘要

本试验研究了不同剂量的鲶鱼垂体提取物(CPGE)对固定潜伏期非洲鲶鱼(Clarias gariepinus)卵和孵化量的影响。本实验采用雌性2 mg/kg、3 mg/kg、4 mg/kg、5 mg/kg 4种剂量。将每只雄性提取的剂量(300 g ~ 305 g)注射到雌性个体(350 g ~ 355 g),分3个重复,共注射12只雌性。在26℃~ 28℃条件下,17小时后将雌虫染纹,计数孵卵。在26℃~ 28℃条件下孵育24小时,计数并记录所有雏鸟。结果表明,各添加量对卵和孵化量有显著差异(P<0.05)。剂量3 (4 mg/kg)与其他处理相比,蛋重、蛋数和蛋重率显著(P<0.05)提高。条纹母鱼的总孵化率和孵化率显著高于其他剂量(P<0.05)。综上所述,4 mg/kg雌虫可获得较高的卵数和等潜伏期的孵化量。因此,为了更好地诱导非洲鲶鱼排卵,建议按本研究建议的剂量使用4 mg/kg雌性脑垂体提取物。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Effects of Different Catfish Pituitary Gland Extract Dosages on Eggs and Hatchlings Quantity of African Catfish, Clarias gariepinus at a Constant Latency Period
An experiment was conducted to assess the effects of different dosages of catfish pituitary glands extract (CPGE) on eggs and hatchlings quantity of African catfish, Clarias gariepinus at a constant latency period. In this experiment, four dosages (2 mg/kg, 3 mg/kg, 4 mg/kg and 5 mg/kg of female) were used. Each dosage extracted from a male (300 g to 305 g) was injected to individual female (350 g to 355 g) at three replications and a total of 12 females were injected. The females were striped after 17 hour at 26°C to 28°C, eggs were counted and incubated. After for 24 hours of incubation at 26°C to 28°C, all hatchlings were counted and recorded. The results indicated that there were significant differences (P<0.05) among the dosages on egg and hatchlings quantity. Egg weight, egg number and percentage egg weight were significantly (P<0.05) higher in dosage three (4 mg/kg) compared to other treatments. Total hatchlings and percentage hatchlings produced from striped females were significantly (P<0.05) higher compared to other dosages. In conclusion, the experiment indicated that higher eggs and hatchlings quantity at a constant latency period are attained with 4 mg/kg of female. Therefore, it is recommended that for better induction of ovulation in African catfish, 4 mg/kg of female pituitary gland extract should be used as suggested by this study.
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