{"title":"聚丙烯酰胺凝胶电泳法鉴定独眼虫属(Heuningbostee)","authors":"O. T. Villiers, M. Bosman, J. H. Lange","doi":"10.1080/02571862.1997.10635081","DOIUrl":null,"url":null,"abstract":"Protein banding patterns of seed obtained by sodium dodecyl sulphate Polyacrylamide get electrophoresis (SDS-PAGE) or acid polycrylamide gel electrophoresis (A-PAGE) were used to distinguish between different Cyclopia species (‘heuningbostee’). An electrophoretic formula was determined for each species using relative band mobility and the number of protein bands Distinctive band patterns were obtained enabling the species to be identified using either SDS-PAGE or A-PAGE Banding patterns showed that this technique can be used as a tool for taxonomic classification of Cyclopia species.","PeriodicalId":22913,"journal":{"name":"The South African Journal of Plant and Soil","volume":"101 1","pages":"54-57"},"PeriodicalIF":0.0000,"publicationDate":"1997-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"1","resultStr":"{\"title\":\"Identification of Cyclopia species (‘Heuningbostee’) using Polyacrylamide gel electrophoresis\",\"authors\":\"O. T. Villiers, M. Bosman, J. H. Lange\",\"doi\":\"10.1080/02571862.1997.10635081\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Protein banding patterns of seed obtained by sodium dodecyl sulphate Polyacrylamide get electrophoresis (SDS-PAGE) or acid polycrylamide gel electrophoresis (A-PAGE) were used to distinguish between different Cyclopia species (‘heuningbostee’). An electrophoretic formula was determined for each species using relative band mobility and the number of protein bands Distinctive band patterns were obtained enabling the species to be identified using either SDS-PAGE or A-PAGE Banding patterns showed that this technique can be used as a tool for taxonomic classification of Cyclopia species.\",\"PeriodicalId\":22913,\"journal\":{\"name\":\"The South African Journal of Plant and Soil\",\"volume\":\"101 1\",\"pages\":\"54-57\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"1997-01-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"1\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"The South African Journal of Plant and Soil\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.1080/02571862.1997.10635081\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"The South African Journal of Plant and Soil","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1080/02571862.1997.10635081","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
Identification of Cyclopia species (‘Heuningbostee’) using Polyacrylamide gel electrophoresis
Protein banding patterns of seed obtained by sodium dodecyl sulphate Polyacrylamide get electrophoresis (SDS-PAGE) or acid polycrylamide gel electrophoresis (A-PAGE) were used to distinguish between different Cyclopia species (‘heuningbostee’). An electrophoretic formula was determined for each species using relative band mobility and the number of protein bands Distinctive band patterns were obtained enabling the species to be identified using either SDS-PAGE or A-PAGE Banding patterns showed that this technique can be used as a tool for taxonomic classification of Cyclopia species.