Determination of Pinoresinol 4-O-beta-D-glucopyranoside and Vladinol F as Markers in Pandanus tonkinensis Fruits by High Performance Liquid Chromatography

According to the traditional know-how, the Northern wild pineapple (Pandanus tonkinensis) has been used efficiently as a major component of oriental remedies for liver protection. Based on the experimentally found chemical composition of Pandanus tonkinensis and their obtained biological activities in terms of the hepatoprotection purpose, two structure-elucidated compounds pinoresinol 4-O-beta-D-glucopyranoside and vladinol F were selected as potential markers for this medical plant. This paper is aiming to present the validation of proposed HPLC method for simultaneous quantification of the both pinoresinol 4-O-beta-D-glucopyranoside and vladinol F in the fruits of Pandanus tonkinensis with the following conditions: using RP-C18 (250 mm × 4.6 mm; 5 μm) column, detection wavelength measured at 228 nm, the mobile phases consisting of acetonitrile and 0.1% acetic acid in gradient mode with a flow rate of 1mL/min and injection volume was 10 mL. Method validation showed a high specificity, linear calibration ranging from 25.5 to 76.4 mg/ml (r= 0.9991) and from 26.0 to 77.9 mg/ml (r= 0.9987) for two markers, respectively. Besides, a good repeatability and intermediate precisions (RSD < 2%), a reliable accuracy (recovery efficiency between 99.4 and 101.5%, RSD < 1.73%) as well as the obtained limits of quantification in values of 2.55 m/g and 2.60 mg/g for the two mentioned markers were received. Using the above validated method, the content of two markers - pinoresinol 4-O-beta-D-glucopyranoside and vladinol F in Pandanus tonkinensis fruits collected from different locations (Thanh Hoa, Hoa Binh, Thai Nguyen) had been determined falling in the range of 0.0250 - 0.0435 mg/g and 0.0243 - 0.0371 mg/g, respectively. Keywords: pinoresinol 4-O-beta-D-glucopyranoside, vladinol F, markers, Pandanus tonkinensis, HPLC