Noer Arif Hardi, Hasan Basri Jumin, Maizar
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摘要

这项研究是在北干巴鲁廖内伊斯兰大学农业学院的生物技术实验室进行的。这项研究从2017年1月到3月进行了5个月。本研究的目的是在体外研究兰蝴蝶植物的生长反应对NAA与椰子水相互作用的影响。本研究采用完全随机设计(RAL)因子,RAL(随机化)由两个因素组成。第一个因子是NAA,由0、0.1 ppm、1.0 ppm和10、0 ppm 4个水平组成;第二个因子是椰子水,也由30%、60%和90% 4个水平组成。重复3次,观察外植体活植率(%)、外植体成芽率(%)、外植体数量、成根率(%)、外植体愈伤组织(%)、茎龄(日)、外植体成苗率(%)。对最后一次观察资料进行统计分析,并以5%的水平继续进行BNJ高级试验。结果表明,NAA与椰子水交互作用对外植体根率和外植体愈伤组织率均有影响,其中NAA浓度为10 ppm时效果最佳。椰子水浓度为30% /L时,对蛾兰的芽数、外植体离根出芽率、外植体成苗率均有显著影响。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
RESPON PERTUMBUHAN EKSPLAN ANGGREK BULAN (Phalaenopsis amabilis. L) TERHADAP PEMBERIAN NAPHTALENE ACETIC ACID (NAA) DAN AIR KELAPA SECARA IN VITRO
This study was conducted in the biotechnology laboratory of the Faculty of Agriculture, Riau Islamic University, Pekanbaru. This study was conducted for five months from January to March 2017. The purpose of this study was to examine the effect of the growth response of an orchid butterfly plant on the interaction between NAA and coconut water using In-Vitro. This study used a Completely Randomized Design (RAL) factorial  the RAL (Randomized ich consists of two factors. The first factor was NAA consisting of 4 levels, i.e 0, 0.1 ppm, 1.0 ppm, and 10,0 ppm, and the second factor was Coconut Water which also consisted of 4 levels, i.e 30%, 60%, and 90%. With 3 repetitions, the parameters observed: percentage of live explants (%), percentage of explants form shoots (%), number, percentage of explants that make up the root (%), explant form callus (%), shoot age (day), and the percentage of explant form plantlets (%). The last observational data were statistically analyzed and continued with BNJ advanced test at a 5% level. As a result, the interaction of NAA and coconut water affected the percentage of explant root and the percentage of explant form callus with the best concentration of 10 ppm NAA. The provision of coconut water had a significant effect on the number of shoots, the percentage of an explant from the roots shoot emergence, explant percentage forming plantlets on moth orchids with a concentration of 30% Coconut water/L media.
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