用激光傅立叶全息显微镜进行三维衍射有限成像

V. Karpov
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引用次数: 0

摘要

对激光全息显微镜(LHM)进行了实验研究。研究了万能副蛔虫(Parascaris Univalens Iarva)标准载玻片。用LHM和高性能尼康常规光学显微镜(COM) 10×100/1.25(浸没油和绿色滤光片)对同一个蛔虫细胞的图像进行了比较,结果表明,这两种显微镜都能提供衍射受限的三维空间分辨率,但对比度差异很大。因此,LHM提供了更多的亚细胞信息。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
3D Diffraction – Limited Imaging with a Laser Fourier Holographic Microscope
A laser holographic microscope (LHM) is investigated experimentally. The standard slide of Parascaris Univalens Iarva (ascaris) is studied. Comparison of the pictures of the same ascaris cell, observed by the LHM and high-performance Nikon conventional optical microscope (COM) 10×100/1.25 with immersion oil and green filter indicates that the both microscopes provide diffraction – limited 3-D spatial resolution, but dramatically different contrast. Thus, the LHM gives much more subcellular information.
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