Chromatographic Analysis of Vitamin B1, B2, B6 and Folic Acid in Multivitamin Pharmaceutical Dosages Available in Local Market

Abstract—In the present study, the RP-HPLC based separation and quantification of four common vitamins: B1, B2, B6, and folic acid in multivitamin pharmaceutical dosages was performed. Simultaneous determination of these vitamins was performed usin Promosil HPLC column C18 at 35 oC with methanol – 0.05 M sodium acid phosphate (80 + 20, v/v) as mobile phase at pH 7.0 using UV-Visible detection system at wavelength 254 nm. Sample selection was made on the basis of products that declare the presence and mount of B group vitamins on their labels. All pharmaceutical dosages confirmed the presence of these vitamins. Amount of these vitamins in all formulations were same as declared on label. All the multivitamin pharmaceutical dosages complied with the standard declaration.