Development of Solid Phase Microextraction-High Performance Liquid Chromatographic Method for the Determination of Copper(II) in Environmental Samples Using Morpholine-4-Carbodithioate

A highly sensitive solid phase microextraction – high performance liquid chromatographic method has been developed for the determination of copper(II) using morpholine-4-carbodithioate (MDTC) as an analytical reagent. The MDTC formed yellowish- brown species of copper(II) at a pH range of 2-9 and complex was sorbed onto PDMS fiber. The Cu(II)-MDTC complex shows maximum absorbance at 430 nm and can be separated on C18 column by using acetonitrile : water (80 : 20) at a flow rate of 1.0 ml min^-1.The detection limit of method was found to be 0.51 μg L^-1. The interference due to common metal ions were studied. The repeatability of the method was checked by finding the relative standard deviation. The developed method was successfully employed for the determination of copper(II) in different alloy samples and the results were compared with existing methods.