M. Rao, V. Devi, C. Sreedevi, T. Rao, C. Chowdary, P. Annapurna
{"title":"鸡禽致病性大肠杆菌毒力基因的分子检测","authors":"M. Rao, V. Devi, C. Sreedevi, T. Rao, C. Chowdary, P. Annapurna","doi":"10.5455/ijlr.20220601053400","DOIUrl":null,"url":null,"abstract":"In the present study, 86 avian pathogenic E. coli isolates from chicken were subjected to multiplex PCR to detect the presence of virulence genes stx1, stx2, eaeA, and hlyA. Forty-seven isolates harbored one or more virulence genes, stx1, stx2, and eaeA while gene hlyA was not detected in any of the samples. Of these, 15 (17.44%) isolates harbored stx1gene, 11 (12.79%) isolates carried stx2 gene, 18 (20.93%) isolates were positive for both stx1 and stx2 genes and 3 (3.49%) isolates carried eaeA gene.","PeriodicalId":14069,"journal":{"name":"International Journal of Livestock Research","volume":"1 1","pages":""},"PeriodicalIF":0.0000,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"1","resultStr":"{\"title\":\"Molecular Detection of Certain Virulence Genes of Avian Pathogenic Escherichia coli (APEC) in Chicken\",\"authors\":\"M. Rao, V. Devi, C. Sreedevi, T. Rao, C. Chowdary, P. Annapurna\",\"doi\":\"10.5455/ijlr.20220601053400\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"In the present study, 86 avian pathogenic E. coli isolates from chicken were subjected to multiplex PCR to detect the presence of virulence genes stx1, stx2, eaeA, and hlyA. Forty-seven isolates harbored one or more virulence genes, stx1, stx2, and eaeA while gene hlyA was not detected in any of the samples. Of these, 15 (17.44%) isolates harbored stx1gene, 11 (12.79%) isolates carried stx2 gene, 18 (20.93%) isolates were positive for both stx1 and stx2 genes and 3 (3.49%) isolates carried eaeA gene.\",\"PeriodicalId\":14069,\"journal\":{\"name\":\"International Journal of Livestock Research\",\"volume\":\"1 1\",\"pages\":\"\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2022-01-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"1\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"International Journal of Livestock Research\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.5455/ijlr.20220601053400\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"International Journal of Livestock Research","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.5455/ijlr.20220601053400","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
Molecular Detection of Certain Virulence Genes of Avian Pathogenic Escherichia coli (APEC) in Chicken
In the present study, 86 avian pathogenic E. coli isolates from chicken were subjected to multiplex PCR to detect the presence of virulence genes stx1, stx2, eaeA, and hlyA. Forty-seven isolates harbored one or more virulence genes, stx1, stx2, and eaeA while gene hlyA was not detected in any of the samples. Of these, 15 (17.44%) isolates harbored stx1gene, 11 (12.79%) isolates carried stx2 gene, 18 (20.93%) isolates were positive for both stx1 and stx2 genes and 3 (3.49%) isolates carried eaeA gene.
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