小孢子虫病的现代诊断

S. Lavrushko, V. Stepanenko
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引用次数: 0

摘要

目的:建立一种基于聚合酶链反应(PCR)的儿童小孢子虫现代分子遗传学诊断方法,以便在DNA水平上鉴定犬小孢子虫病原。材料和方法。本研究包括40例皮肤光滑、头皮光滑、头皮和皮肤光滑的小孢子虫。本研究的生物材料为小孢子虫患者光滑皮肤和头皮上的鳞片、头皮上的毛发。对皮肤光滑小孢子虫、头皮小孢子虫、头皮小孢子虫和皮肤光滑小孢子虫患者的40份生物材料样本进行了研究。第一阶段对犬小孢子菌进行DNA分离。然后用特定引物进行PCR扩增DNA区域的拷贝数。最后一步是输入40个患者临床资料样本。结果和讨论。PCR诊断可以在所有40份小孢子虫患者的生物材料样本中鉴定出犬小孢子虫的DNA。在我们的研究中,我们开发了一种基于pcr的诊断小孢子虫的方法,该方法使用一组两个MC引物(犬小孢子菌β微管蛋白基因区域)。为了内部控制扩增过程和生物材料取样的质量,还使用了APOE(人类载脂蛋白E基因的一个区域)的特定引物。结论。为了提高儿童小孢子虫的精确特异性诊断,建立了一种基于聚合酶链反应(PCR)的现代分子遗传学诊断方法,可以在DNA水平上对犬小孢子虫病原体进行鉴定。对犬小孢子虫基因组的分子结构分析表明,PCR是最客观的微生物诊断方法。该方法可应用于人类犬小孢子虫的检测算法中。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Modern diagnostics of microsporia
Objective — to develop a method of modern molecular genetic diagnosis of microsporia in children based on polymerase chain reaction (PCR), which will allow identification of the pathogen of Microsporum canis at the DNA level. Materials and methods. The study included 40 patients with microsporia of smooth skin, scalp, scalp and smooth skin. The biological materials for the research were scales from the smooth skin and scalp, hair from the scalp of patients with microsporia. A study of 40 samples of biological material was carried out in patients with microsporia of smooth skin, microsporia of the scalp, microsporia of the scalp and smooth skin. At the first stage, DNA isolation of Microsporum canis was carried out. Then PCR was carried out to increase the copies of the DNA region using specific primers. The final step was typing 40 samples of clinical material of patients. Results and discussion. PCR diagnostics made it possible to identify the DNA of Microsporum canis in all 40 samples of biological material of patients with microsporia. In our study, we developed a PCR-based method for diagnosing microsporia, which uses a set of two MC primers (regions of the beta tubulin gene of Microsporum canis). For internal control of the course of amplification and the quality of biomaterial sampling, specific primers of APOE (a region of the human apolipoprotein E gene) were also used. Conclusions. In order to improve the precise specific diagnosis of microsporia in children, a method of modern molecular genetic diagnostics based on polymerase chain reaction (PCR) has been developed, which allows identification of the Microsporum canis pathogen at the DNA level. Analysis of the molecular structure of the genome of Microsporum canis proved that the most objective diagnosis of microorganisms is the PCR method. The developed method of DNA diagnostics based on PCR using specific primers can be included in the algorithm for detecting Microsporum canis in humans.
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