透射电子显微镜在评估细菌细胞和微生物种群质量方面的可能性。

Q4 Health Professions

Klinichescheskaya Laboratornaya Diagnostika Pub Date : 2022-04-17 DOI:10.51620/0869-2084-2022-67-4-237-243

V. N. Gerasimov, N. N. Marinina, G. G. Kharseeva, O. S. Sherbataya

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引用次数: 2

摘要

采用透射电子显微镜超薄切片可视化的方法，测定抗生素、消毒剂、生物素、物理和化学制剂对微生物形态结构的影响。目的是开发在透射电子显微镜下快速制备和可视化细菌超微结构的方法学技术，以检测细胞的结构损伤并评估微生物种群的质量。本文提出了一种在透射电子显微镜下进行细菌精细结构可视化和细胞结构损伤诊断的加速样品制备技术。使用这种方法将有可能更有效地研究微生物在接触抗生素、消毒剂、生物制剂的不同阶段的失活机制，包括各种紧张的、破坏性的和致命的物理和化学效应。

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Possibilities of transmission electron microscopy in assessing the quality of bacterial cells and microbial population.

Determination of changes in the morphostructure of microorganisms under the influence of antibiotics, disinfectants, biocins, physical and chemical agents is carried out using the method of visualization of ultrathin sections in a transmission electron microscope. The aim is to develop methodological techniques for express preparation and visualization of the ultrastructure of bacteria in a transmission electron microscope to detect structural damage to cells and assess the quality of the microbial population. A technique of accelerated sample preparation for visualization of the fine structure of bacteria and diagnosis of structural damage to cells in a transmission electron microscope has been developed. The use of this method will make it possible to more effectively investigate the mechanisms of inactivation of microorganisms at different stages of contact with antibiotics, disinfectants, biocines, including various stressful, damaging and lethal physical and chemical effects.

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来源期刊

Klinichescheskaya Laboratornaya Diagnostika Health Professions-Medical Laboratory Technology

CiteScore

0.90

自引率

0.00%

发文量

110

期刊介绍： The journal deals with theoretical and practical problems of clinical laboratory diagnosis, publishes editorial articles, reviews of literature, original articles, short reports, discussions, book reviews, current events, materials which may assist the practitioners, methods of laboratory investigations used in medicine, materials on the results of practical application of new methods of investigation in the following fields of clinical laboratory diagnosis: hematology, cytology, coagulation, biochemistry, immunology.