利用随机扩增多态DNA评估阿卡尼水杨的遗传特征

IF 2.1 4区生物学 Q2 Agricultural and Biological Sciences

Caryologia Pub Date : 2022-09-21 DOI:10.36253/caryologia-1493

Zhu Lin, H. Khodayari

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引用次数: 0

摘要

盐角草是一种野生的一年生盐生植物(某些种类为两年生)，来自藜科，生长在亚洲、北美和中东的盐沼和盐渍湿地附近或沿海地区。它也生长在伊朗的半干旱地区，如伊斯法罕、法尔斯和亚兹德省。对10个地理居群的遗传变异和居群结构进行了研究。测定了这些群体的遗传多样性参数。20条随机扩增多态性DNA (RAPD)引物中，10条引物产生146条可重复条带，平均14.6条，多态性率为88.67%。OPA10引物有效等位基因数(Ne)、Shannon指数(I)和遗传多样性(H)最高，RAPD标记遗传多样性最高的是Esfahan和Nain，距离Varzaneh群体70km。RAPD数据的WARD树将群体分为两个不同的聚类/组，表明了它们的遗传差异，并对此进行了详细的讨论。本研究结果表明，海角的遗传变异水平相对较低。基于ward的树形图显示Esfahan, Varzaneh和Esfahan (Varzaneh的Zayanderud河)成员之间的关系密切，而Yazd, Meybud和Yazd, Nádüshan受保护种群与其他种群差异最大。然而，主成分分析显示了与基于ward的树突图的一些细微差异。

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Genetic Characterization of Salicornia persica Akhani (Chenopodiaceae) Assessed Using Random Amplified Polymorphic DNA

Salicornia is a wild and annual (biennial, in some species) halophytic plant from Chenopodiaceae family that grows near salt marshes and salted wetlands or the vicinity of coastal areas in Asia, North America, and the Middle East. It is also grown in semi-arid areas of Iran such as Isfahan, Fars, and Yazd provinces. Genetic variability and populations, structure were studied in 10 geographical populations of Salicornia persica. Genetic diversity parameters were determined in these populations. 10 of 20 random amplified polymorphic DNA (RAPD) primers produced 146 reproducible bands with average of 14.6 bands per primer and 88.67% of polymorphism. OPA10 primer showed the highest number of effective allele (Ne), Shannon index (I) and genetic diversity (H). The highest values of genetic diversity in RAPD markers were obtained in Esfahan, Nain, it is 70km to Varzaneh populations. WARD trees of RAPD data grouped the populations in two different clusters/groups, indicating their genetic difference which is discussed in details. The results of this study showed that the level of genetic variation in Salicornia persica is relatively low. WARD-based dendrogram showed a close relationship between members of Esfahan, Varzaneh and Esfahan, the river of Zayanderud at Varzaneh while the Yazd, Meybud and Yazd, Nádüshan protected population differ the most from the other populations. Principal component analysis, however, showed some minor differences with WARD-based dendrograms.

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来源期刊

Caryologia 生物-遗传学

CiteScore

1.60

自引率

23.80%

发文量

审稿时长

12 months

期刊介绍： Caryologia is devoted to the publication of original papers, and occasionally of reviews, about plant, animal and human karyological, cytological, cytogenetic, embryological and ultrastructural studies. Articles about the structure, the organization and the biological events relating to DNA and chromatin organization in eukaryotic cells are considered. Caryologia has a strong tradition in plant and animal cytosystematics and in cytotoxicology. Bioinformatics articles may be considered, but only if they have an emphasis on the relationship between the nucleus and cytoplasm and/or the structural organization of the eukaryotic cell.