荧光原位杂交技术

Nigel P Carter
{"title":"荧光原位杂交技术","authors":"Nigel P Carter","doi":"10.1002/1361-6374(199606)4:2<41::AID-BIO2>3.0.CO;2-X","DOIUrl":null,"url":null,"abstract":"<p>Fluorescence <i>in situ</i> hybridization (FISH) has become a widely used technique for the cytogenetic analysis of cells and genomes. Today, FISH techniques allow DNA and RNA probes to be localized in tissue or cellular preparations with speed, specificity, simplicity and safety. Improvements in probe labelling and detection, fluorescence microscopy and the introduction of digital imaging have all combined to enable FISH to be applied not only to answer questions about genome organization, structure and function but also to play an important diagnostic role in the clinical laboratory.</p>","PeriodicalId":100176,"journal":{"name":"Bioimaging","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"1996-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1002/1361-6374(199606)4:2<41::AID-BIO2>3.0.CO;2-X","citationCount":"18","resultStr":"{\"title\":\"Fluorescence in situ hybridization—state of the art\",\"authors\":\"Nigel P Carter\",\"doi\":\"10.1002/1361-6374(199606)4:2<41::AID-BIO2>3.0.CO;2-X\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p>Fluorescence <i>in situ</i> hybridization (FISH) has become a widely used technique for the cytogenetic analysis of cells and genomes. Today, FISH techniques allow DNA and RNA probes to be localized in tissue or cellular preparations with speed, specificity, simplicity and safety. Improvements in probe labelling and detection, fluorescence microscopy and the introduction of digital imaging have all combined to enable FISH to be applied not only to answer questions about genome organization, structure and function but also to play an important diagnostic role in the clinical laboratory.</p>\",\"PeriodicalId\":100176,\"journal\":{\"name\":\"Bioimaging\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":0.0000,\"publicationDate\":\"1996-06-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://sci-hub-pdf.com/10.1002/1361-6374(199606)4:2<41::AID-BIO2>3.0.CO;2-X\",\"citationCount\":\"18\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Bioimaging\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://onlinelibrary.wiley.com/doi/10.1002/1361-6374%28199606%294%3A2%3C41%3A%3AAID-BIO2%3E3.0.CO%3B2-X\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Bioimaging","FirstCategoryId":"1085","ListUrlMain":"https://onlinelibrary.wiley.com/doi/10.1002/1361-6374%28199606%294%3A2%3C41%3A%3AAID-BIO2%3E3.0.CO%3B2-X","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 18

摘要

荧光原位杂交(FISH)已成为一种广泛用于细胞和基因组细胞遗传学分析的技术。如今,FISH技术使DNA和RNA探针能够快速、特异、简单和安全地定位在组织或细胞制剂中。探针标记和检测、荧光显微镜的改进以及数字成像的引入,使FISH不仅可以应用于回答有关基因组组织、结构和功能的问题,而且可以在临床实验室中发挥重要的诊断作用。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Fluorescence in situ hybridization—state of the art

Fluorescence in situ hybridization (FISH) has become a widely used technique for the cytogenetic analysis of cells and genomes. Today, FISH techniques allow DNA and RNA probes to be localized in tissue or cellular preparations with speed, specificity, simplicity and safety. Improvements in probe labelling and detection, fluorescence microscopy and the introduction of digital imaging have all combined to enable FISH to be applied not only to answer questions about genome organization, structure and function but also to play an important diagnostic role in the clinical laboratory.

求助全文
通过发布文献求助,成功后即可免费获取论文全文。 去求助
来源期刊
自引率
0.00%
发文量
0
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信