荧光原位杂交技术

Nigel P Carter
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引用次数: 18

摘要

荧光原位杂交(FISH)已成为一种广泛用于细胞和基因组细胞遗传学分析的技术。如今,FISH技术使DNA和RNA探针能够快速、特异、简单和安全地定位在组织或细胞制剂中。探针标记和检测、荧光显微镜的改进以及数字成像的引入,使FISH不仅可以应用于回答有关基因组组织、结构和功能的问题,而且可以在临床实验室中发挥重要的诊断作用。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Fluorescence in situ hybridization—state of the art

Fluorescence in situ hybridization (FISH) has become a widely used technique for the cytogenetic analysis of cells and genomes. Today, FISH techniques allow DNA and RNA probes to be localized in tissue or cellular preparations with speed, specificity, simplicity and safety. Improvements in probe labelling and detection, fluorescence microscopy and the introduction of digital imaging have all combined to enable FISH to be applied not only to answer questions about genome organization, structure and function but also to play an important diagnostic role in the clinical laboratory.

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