突变小鼠LacZ转基因定位于3号染色体

Mutation Research Letters Pub Date : 1994-12-01 DOI:10.1016/0165-7992(94)90077-9

Roy R. Swiger , Brian Myhr , James D. Tucker

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引用次数: 20

摘要

转基因小鼠模型越来越频繁地用于突变和毒理学研究。一个这样的系统，MutaMouse，在小鼠基因组中包含稳定整合的lambda-gt10LacZ穿梭载体。我们描述了使用双色荧光原位杂交（FISH）与Mus musculus全染色体涂料和lambda DNA来将lambda转基因的整合位点定位到小鼠3号染色体上的C带。

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The LacZ transgene in mutaTM Mouse maps to chromosome 3

Transgenic mouse models are being used with increasing frequency for mutational and toxicological studies. One such system, MutaMouse, contains a stably integrated lambda-gt10LacZ shuttle vector in the mouse genome. We describe the use of dual color fluorescence in situ hybridization (FISH) with Mus musculus whole chromosome paints and lambda DNA to map the integration site of the lambda transgene to band C on mouse chromosome 3.

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Mutation Research Letters

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