Cascade-Cas3利用噬菌体编码的同源重组促进假单胞菌的高精度基因组工程

Engineering Microbiology Pub Date : 2022-12-01 DOI:10.1016/j.engmic.2022.100046

Wentao Zheng , Yandong Xia , Xue Wang , Shiqing Gao , Diao Zhou , Jun Fu , Ruijuan Li , Jia Yin

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引用次数: 4

摘要

噬菌体编码同源重组（PEHR）是细菌基因组编辑的有效工具。我们之前开发并使用了假单胞菌特异性PEHR系统。然而，当使用PEHR系统进行假单胞菌基因组编辑时，假阳性可能是一个问题。在本研究中，我们将来自铜绿假单胞菌（PaeCas3c）的紧凑型Cascade-Cas3系统与假单胞菌特异性PEHR系统相结合，我们的重组分析结果表明，这种紧凑型Cascas3系统可以显著提高PEHR重组的准确性。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

Cascade-Cas3 facilitates high-accuracy genome engineering in Pseudomonas using phage-encoded homologous recombination

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Cascade-Cas3 facilitates high-accuracy genome engineering in Pseudomonas using phage-encoded homologous recombination

Phage-encoded homologous recombination (PEHR) is an efficient tool for bacterial genome editing. We previously developed and utilized a Pseudomonas-specific PEHR system. However, when using the PEHR system for Pseudomonas genome editing, false positives can be a problem. In this study, we combined a compact Cascade-Cas3 system from P. aeruginosa (PaeCas3c) with a Pseudomonas-specific PEHR system, and the results of our recombineering assay showed that this compact Cascade-Cas3 system can significantly improve PEHR recombineering accuracy.

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来源期刊

Engineering Microbiology

CiteScore

3.90

自引率

0.00%

发文量