{"title":"利用SP6聚合酶和兔网织细胞裂解液高效体外表达干扰素α类似物","authors":"Martin J. Tymms, Beth McInnes","doi":"10.1016/0735-0651(88)90021-0","DOIUrl":null,"url":null,"abstract":"<div><p>We have investigated the use of in vitro expression as a quick and convenient means of screening large numbers of interferon (IFN) analogs generated using in vitro mutagenesis. The IFN-α1 mRNA generated from DNA template using SP6 RNA polymerase is efficiently translated in rabbit reticulocyte lysate (RRL). The antiviral specific activity of this RRL-synthesized IFN-α1 is equivalent to the yeast-synthesized protein. In contrast with the yeast-expression system, where some IFN-α analogs are poorly expressed, all analogs tested were well expressed in RRL.</p></div>","PeriodicalId":77714,"journal":{"name":"Gene analysis techniques","volume":"5 1","pages":"Pages 9-15"},"PeriodicalIF":0.0000,"publicationDate":"1988-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0735-0651(88)90021-0","citationCount":"25","resultStr":"{\"title\":\"Efficient in vitro expression of interferon α analogs using SP6 polymerase and rabbit reticulocyte lysate\",\"authors\":\"Martin J. Tymms, Beth McInnes\",\"doi\":\"10.1016/0735-0651(88)90021-0\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>We have investigated the use of in vitro expression as a quick and convenient means of screening large numbers of interferon (IFN) analogs generated using in vitro mutagenesis. The IFN-α1 mRNA generated from DNA template using SP6 RNA polymerase is efficiently translated in rabbit reticulocyte lysate (RRL). The antiviral specific activity of this RRL-synthesized IFN-α1 is equivalent to the yeast-synthesized protein. In contrast with the yeast-expression system, where some IFN-α analogs are poorly expressed, all analogs tested were well expressed in RRL.</p></div>\",\"PeriodicalId\":77714,\"journal\":{\"name\":\"Gene analysis techniques\",\"volume\":\"5 1\",\"pages\":\"Pages 9-15\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"1988-01-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://sci-hub-pdf.com/10.1016/0735-0651(88)90021-0\",\"citationCount\":\"25\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Gene analysis techniques\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/0735065188900210\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Gene analysis techniques","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/0735065188900210","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
Efficient in vitro expression of interferon α analogs using SP6 polymerase and rabbit reticulocyte lysate
We have investigated the use of in vitro expression as a quick and convenient means of screening large numbers of interferon (IFN) analogs generated using in vitro mutagenesis. The IFN-α1 mRNA generated from DNA template using SP6 RNA polymerase is efficiently translated in rabbit reticulocyte lysate (RRL). The antiviral specific activity of this RRL-synthesized IFN-α1 is equivalent to the yeast-synthesized protein. In contrast with the yeast-expression system, where some IFN-α analogs are poorly expressed, all analogs tested were well expressed in RRL.
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