{"title":"生物源性金属纳米颗粒作为细胞代谢活性指标的从头生成","authors":"D. A. Skladnev, V. V. Sorokin","doi":"10.1134/S2635167623700313","DOIUrl":null,"url":null,"abstract":"<p>The dynamics of the de novo generation of biogenic metal nanoparticles clearly reflects the features of the interaction of the used cells with cations. With this taken into account, we develop a methodological approach that makes it possible to assess the level of metabolic activity of cells by measuring the linear parameters of biogenic metal nanoparticles that are formed in situ in minutes (DBNG, detection of biogenic-nanoparticle generation/growth). This work shows examples of the practical use of this research approach, which makes it possible to detect the contamination of growth media, assess the physiological state of pure cultures of microorganisms during their cultivation, compare the physiological state of natural microbial communities under changing environmental conditions, identify metabolic differences when comparing genetically closely related cultures of microorganisms, search for cultures with an increased level of target metabolic activities for practical use, and differentiate types of human tumor tissues.</p>","PeriodicalId":716,"journal":{"name":"Nanotechnologies in Russia","volume":null,"pages":null},"PeriodicalIF":0.8000,"publicationDate":"2023-09-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"De Novo Generation of Biogenic Metal Nanoparticles As an Indicator of Cell Metabolic Activity\",\"authors\":\"D. A. Skladnev, V. V. Sorokin\",\"doi\":\"10.1134/S2635167623700313\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p>The dynamics of the de novo generation of biogenic metal nanoparticles clearly reflects the features of the interaction of the used cells with cations. With this taken into account, we develop a methodological approach that makes it possible to assess the level of metabolic activity of cells by measuring the linear parameters of biogenic metal nanoparticles that are formed in situ in minutes (DBNG, detection of biogenic-nanoparticle generation/growth). This work shows examples of the practical use of this research approach, which makes it possible to detect the contamination of growth media, assess the physiological state of pure cultures of microorganisms during their cultivation, compare the physiological state of natural microbial communities under changing environmental conditions, identify metabolic differences when comparing genetically closely related cultures of microorganisms, search for cultures with an increased level of target metabolic activities for practical use, and differentiate types of human tumor tissues.</p>\",\"PeriodicalId\":716,\"journal\":{\"name\":\"Nanotechnologies in Russia\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":0.8000,\"publicationDate\":\"2023-09-19\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Nanotechnologies in Russia\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://link.springer.com/article/10.1134/S2635167623700313\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q3\",\"JCRName\":\"Engineering\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Nanotechnologies in Russia","FirstCategoryId":"1085","ListUrlMain":"https://link.springer.com/article/10.1134/S2635167623700313","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"Engineering","Score":null,"Total":0}
De Novo Generation of Biogenic Metal Nanoparticles As an Indicator of Cell Metabolic Activity
The dynamics of the de novo generation of biogenic metal nanoparticles clearly reflects the features of the interaction of the used cells with cations. With this taken into account, we develop a methodological approach that makes it possible to assess the level of metabolic activity of cells by measuring the linear parameters of biogenic metal nanoparticles that are formed in situ in minutes (DBNG, detection of biogenic-nanoparticle generation/growth). This work shows examples of the practical use of this research approach, which makes it possible to detect the contamination of growth media, assess the physiological state of pure cultures of microorganisms during their cultivation, compare the physiological state of natural microbial communities under changing environmental conditions, identify metabolic differences when comparing genetically closely related cultures of microorganisms, search for cultures with an increased level of target metabolic activities for practical use, and differentiate types of human tumor tissues.
期刊介绍：
Nanobiotechnology Reports publishes interdisciplinary research articles on fundamental aspects of the structure and properties of nanoscale objects and nanomaterials, polymeric and bioorganic molecules, and supramolecular and biohybrid complexes, as well as articles that discuss technologies for their preparation and processing, and practical implementation of products, devices, and nature-like systems based on them. The journal publishes original articles and reviews that meet the highest scientific quality standards in the following areas of science and technology studies: self-organizing structures and nanoassemblies; nanostructures, including nanotubes; functional and structural nanomaterials; polymeric, bioorganic, and hybrid nanomaterials; devices and products based on nanomaterials and nanotechnology; nanobiology and genetics, and omics technologies; nanobiomedicine and nanopharmaceutics; nanoelectronics and neuromorphic computing systems; neurocognitive systems and technologies; nanophotonics; natural science methods in a study of cultural heritage items; metrology, standardization, and monitoring in nanotechnology.
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