多发性骨髓瘤单细胞RNA测序的基本程序及其翻译价值。

IF 1.5 Q3 HEMATOLOGY
血液科学(英文) Pub Date : 2023-11-02 eCollection Date: 2023-10-01 DOI:10.1097/BS9.0000000000000172
Jun Du, Xiao-Ran Gu, Xiao-Xiao Yu, Yang-Jia Cao, Jian Hou
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引用次数: 0

摘要

多发性骨髓瘤(MM)是一种以异常浆细胞克隆增殖为特征的恶性肿瘤。在许多国家,它是造血系统中第二常见的恶性肿瘤。尽管MM的治疗方法不断改进,患者的生存期显著延长,但MM仍然是一种不可治愈的疾病,复发几率很高。因此,仍有许多挑战需要解决。一种有前景的方法是单细胞RNA测序(scRNA-seq),它可以阐明单个细胞的转录组异质性,并揭示复杂组织中以前未知的细胞类型或状态。在这篇综述中,我们概述了scRNA-seq在MM中的实验工作流程,列出了一些常用的scRNA-seq平台和分析工具。此外,随着scRNA-seq的出现,许多研究在MM克隆进化的关键分子机制、微环境中的细胞相互作用和分子调控以及靶向治疗中的耐药性机制等方面取得了新的进展。我们总结了将scRNA-seq应用于MM研究的主要发现和测序平台,并基于这些发现提出了靶向治疗的广泛方向。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Essential procedures of single-cell RNA sequencing in multiple myeloma and its translational value.

Essential procedures of single-cell RNA sequencing in multiple myeloma and its translational value.

Essential procedures of single-cell RNA sequencing in multiple myeloma and its translational value.

Multiple myeloma (MM) is a malignant neoplasm characterized by clonal proliferation of abnormal plasma cells. In many countries, it ranks as the second most prevalent malignant neoplasm of the hematopoietic system. Although treatment methods for MM have been continuously improved and the survival of patients has been dramatically prolonged, MM remains an incurable disease with a high probability of recurrence. As such, there are still many challenges to be addressed. One promising approach is single-cell RNA sequencing (scRNA-seq), which can elucidate the transcriptome heterogeneity of individual cells and reveal previously unknown cell types or states in complex tissues. In this review, we outlined the experimental workflow of scRNA-seq in MM, listed some commonly used scRNA-seq platforms and analytical tools. In addition, with the advent of scRNA-seq, many studies have made new progress in the key molecular mechanisms during MM clonal evolution, cell interactions and molecular regulation in the microenvironment, and drug resistance mechanisms in target therapy. We summarized the main findings and sequencing platforms for applying scRNA-seq to MM research and proposed broad directions for targeted therapies based on these findings.

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来源期刊
CiteScore
1.70
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