偏振显微镜：从整体结构成像到单分子三维定向和定位显微镜

IF 8.4 1区物理与天体物理 Q1 OPTICS

Optica Pub Date : 2023-11-07 DOI:10.1364/optica.502119

Sophie Brasselet and Miguel A. Alonso

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引用次数: 0

摘要

显微镜中的光学对比度对光偏振很敏感，光偏振与分子偶极子的相互作用为探测分子取向提供了重要的杠杆。在过去的十年里，偏振显微镜有了很大的发展，集成了从传统的线性二向色到单分子取向和定位成像的各种策略。这篇综述旨在总结分子水平的取向和结构成像背后的概念和技术，从2D的集成显微镜到3D的单分子超分辨率显微镜。

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Polarization microscopy: from ensemble structural imaging to single-molecule 3D orientation and localization microscopy

Optical contrasts in microscopy are sensitive to light polarization, whose interaction with molecular dipoles provides an important lever for probing molecular orientation. Polarization microscopy has evolved considerably during the last decade, integrating strategies ranging from traditional linear dichroism to single-molecule orientation and localization imaging. This review aims to provide a summary of concepts and techniques behind orientation and structural imaging at the molecular level, from ensemble microscopy in 2D to single-molecule super-resolution microscopy in 3D.

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来源期刊

Optica OPTICS-

CiteScore

19.70

自引率

2.90%

发文量

191

审稿时长

2 months

期刊介绍： Optica is an open access, online-only journal published monthly by Optica Publishing Group. It is dedicated to the rapid dissemination of high-impact peer-reviewed research in the field of optics and photonics. The journal provides a forum for theoretical or experimental, fundamental or applied research to be swiftly accessed by the international community. Optica is abstracted and indexed in Chemical Abstracts Service, Current Contents/Physical, Chemical & Earth Sciences, and Science Citation Index Expanded.