尖叶Alpinia oxyphylla（+）-notkatone生物合成的完整途径阐明和异源重组。

IF 8.3 1区生物学 Q1 PLANT SCIENCES

New Phytologist Pub Date : 2023-11-07 DOI:10.1111/nph.19375

Xiaomin Deng, Ziling Ye, Jingyu Duan, Fangfang Chen, Yao Zhi, Man Huang, Minjian Huang, Weijia Cheng, Yujie Dou, Zhaolin Kuang, Yanglei Huang, Guangkai Bian, Zixin Deng, Tiangang Liu, Li Lu

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引用次数: 0

摘要

（+）-Notkatone是一种天然倍半萜酮，广泛用于食品、化妆品、制药和农业。它也被认为是商业上使用的最有价值的萜烯之一。然而，植物中含有微量的（+）-notkatone，从植物中提取不足以满足市场需求。益智是我国著名的药用植物，（+）-notkatone是其果实中的主要成分之一。通过转录组挖掘和使用提供前体的酵母底盘进行功能筛选，鉴定了益智中完整的（+）-notkatone生物合成途径。（+）-价烯合酶（AoVS）被鉴定为一种新的单子叶植物衍生的价烯合成酶；通过构建提供价烯的酵母菌株鉴定了三种（+）-价烯氧化酶AoCYP6（CYP71BB2）、AoCYP9（CYP71CX8）和AoCYP18（CYP701A170）。通过对细胞色素P450还原酶（AoCPR1）和三种脱氢酶（AoSDR1/2/3）的进一步表征，我们成功地在酿酒酵母中重建了（+）-notkatone生物合成途径，为其生物技术生产奠定了基础。确定了（+）-绞股蓝酮在植物中的生物合成途径，揭示了其在植物中形成的分子机制，也为（+）-Notkatone的生物工程生产提供了支持。高效的酵母底盘筛选方法可用于阐明其他有价值的植物天然产物的完整生物合成途径。

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Complete pathway elucidation and heterologous reconstitution of (+)-nootkatone biosynthesis from Alpinia oxyphylla

(+)-Nootkatone is a natural sesquiterpene ketone widely used in food, cosmetics, pharmaceuticals, and agriculture. It is also regarded as one of the most valuable terpenes used commercially. However, plants contain trace amounts of (+)-nootkatone, and extraction from plants is insufficient to meet market demand. Alpinia oxyphylla is a well-known medicinal plant in China, and (+)-nootkatone is one of the main components within the fruits.
By transcriptome mining and functional screening using a precursor-providing yeast chassis, the complete (+)-nootkatone biosynthetic pathway in Alpinia oxyphylla was identified.
A (+)-valencene synthase (AoVS) was identified as a novel monocot-derived valencene synthase; three (+)-valencene oxidases AoCYP6 (CYP71BB2), AoCYP9 (CYP71CX8), and AoCYP18 (CYP701A170) were identified by constructing a valencene-providing yeast strain. With further characterisation of a cytochrome P450 reductase (AoCPR1) and three dehydrogenases (AoSDR1/2/3), we successfully reconstructed the (+)-nootkatone biosynthetic pathway in Saccharomyces cerevisiae, representing a basis for its biotechnological production.
Identifying the biosynthetic pathway of (+)-nootkatone in A. oxyphylla unravelled the molecular mechanism underlying its formation in planta and also supported the bioengineering production of (+)-nootkatone. The highly efficient yeast chassis screening method could be used to elucidate the complete biosynthetic pathway of other valuable plant natural products in future.

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来源期刊

New Phytologist 生物-植物科学

自引率

5.30%

发文量

728

期刊介绍： New Phytologist is an international electronic journal published 24 times a year. It is owned by the New Phytologist Foundation, a non-profit-making charitable organization dedicated to promoting plant science. The journal publishes excellent, novel, rigorous, and timely research and scholarship in plant science and its applications. The articles cover topics in five sections: Physiology & Development, Environment, Interaction, Evolution, and Transformative Plant Biotechnology. These sections encompass intracellular processes, global environmental change, and encourage cross-disciplinary approaches. The journal recognizes the use of techniques from molecular and cell biology, functional genomics, modeling, and system-based approaches in plant science. Abstracting and Indexing Information for New Phytologist includes Academic Search, AgBiotech News & Information, Agroforestry Abstracts, Biochemistry & Biophysics Citation Index, Botanical Pesticides, CAB Abstracts®, Environment Index, Global Health, and Plant Breeding Abstracts, and others.