再利用药物辛伐他汀/胸腺醌联合治疗乳腺癌症细胞系增强细胞毒性效应的评价。

Pallavi Kumari, Shweta Dang
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引用次数: 0

摘要

引言:在药物发现中,重新利用药物的抗癌潜力变得越来越重要。目的:本研究旨在探索治疗高胆固醇药物辛伐他汀(SIM)和胸腺醌(Nigella Sativa)(THY)对癌症细胞株的抗癌活性。据报道,胸腺醌具有许多潜在的药用特性,表现出抗氧化、抗炎、抗癌以及组织生长和分裂、激素调节、免疫反应和发育以及细胞信号传导等活性。方法:探讨辛伐他汀与胸腺嘧啶醌联合应用对癌症细胞株MCF-7和MDA-MB-231的抑制作用。研究了辛伐他汀和胸腺醌对体外细胞活力、集落形成、细胞迁移和更多程序性细胞死亡方向的联合作用。结果:联合治疗对MCF-7细胞和MDA-MB-231细胞的细胞周期阻滞分别为8.1%和3.8%和MDA-MB-231细胞。结论:辛伐他汀与胸腺嘧啶醌联合作用可促进癌症细胞凋亡。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Evaluation of Enhanced Cytotoxicity Effect of Repurposed Drug Simvastatin/Thymoquinone Combination against Breast Cancer Cell Line.

Introduction: Repurposing of drugs for their anticancer potential is gaining a lot of importance in drug discovery.

Aims: The present study aims to explore the potential of Simvastatin (SIM), a drug used in the treatment of high cholesterol, and Thymoquinone (Nigella Sativa) (THY) for its anti-cancer activity on breast cancer cell lines. Thymoquinone is reported to have many potential medicinal properties exhibiting antioxidant, antiinflammatory, anti-cancer, activities like inhibition of tissue growth and division.

Methods: In this analysis, we explored the inhibitory effects of the combination of Simvastatin ad Thymoquinone on two breast cancer cell lines viz MCF-7 and MDA-MB-231 cells. The combined effect of Simvastatin and Thymoquinone on Cell viability, Colony formation, Cell migration, and orientation of more programmed cell death in vitro was studied. Cell cycle arrest in the G2/M phase was concomitant with the combined effect of SIM and THY persuading apoptosis and generating reactive oxygen species (ROS).

Results: The cell cycle arrest with combined treatment was observed that only 1.8% and 1.1% cells gated in G2/M phase in MCF-7 & MDA-MB-231 cell. An increased apoptosis was observed when cells were treated in combination which was about 76.20% and 58.15% respectively for MCF-7 and MDA-MB-231 cells.

Conclusion: It was concluded that the combined effect of simvastatin and thymoquinone stimulates apoptosis in breast cancer cells.

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