利用半胱天冬酶途径研究东方枫香(金缕梅科)中的枫香对HEp-2癌症细胞的细胞毒性和凋亡作用。

IF 0.9 Q3 MEDICINE, GENERAL & INTERNAL
Tuğçe Duran, Zeliha Tuncer
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引用次数: 0

摘要

目的:首次鉴定HEp-2细胞系为癌症细胞。然后,据报道,它由通过HeLa细胞系污染衍生的宫颈腺癌细胞组成。液体安息香是由东方枫香(金缕梅科)受伤的外壳提供的渗出物,已用于治疗皮肤问题、消化性溃疡和寄生虫感染或用作防腐剂。本研究旨在研究液体苯乙烯对HEp-2癌症细胞系的细胞毒性和凋亡作用。材料与方法:采用(3-(4,5-二甲基噻唑-2-基)-2,5-二苯基溴化四唑啉)法测定液体苯乙烯(土耳其树胶)的IC50剂量,并通过定量实时聚合酶链反应评估参与凋亡的基因(Bax、Bad、Bak1、p53、Bcl-2、Bcl-XL、Apaf-1、Caspase2、Caspase3a、Caspase9和Caspase12)的表达来确定液体苯乙烯对HEp-2癌症细胞的凋亡作用。结果:苯乙烯液相48小时的IC50值为125μg/mL。结果表明,液体苯乙烯减少了HEp-2喉癌细胞的数量,增加了凋亡相关基因的表达。这些结果表明液体苯乙烯可以被认为是癌症治疗的一种选择。结论:本研究结果表明,对液体苯乙烯对癌症细胞的作用进行更为全面的研究将更为有益。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Investigation of Cytotoxic and Apoptotic Effects of Styrax Liquidus Obtained From Liquidambar orientalis Miller (Hamamelidaceae) on HEp-2 Cancer Cell with Caspase Pathway.

Objective: The HEp-2 cell line was first identified as laryngeal cancer cells. Then, it was reported to consist of cervical adenocarcinoma cells derived via HeLa cell line contamination. Styrax liquidus is an exudate that is provided by the injured hull of the Liquidambar orientalis Miller (Hamamelidaceae), which has been used for the treatment of skin problems, peptic ulcers, and parasitic infections or as an antiseptic. In our study, we purposed to research the cytotoxic and apoptotic effect of styrax liquidus on HEp-2 cancer cell line.

Materials and methods: The IC50 dosage of styrax liquidus (Turkish sweet gum obtained from trees) was set by the (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay, and the apoptotic effect of styrax liquidus on HEp-2 cancer cell was determined by assessing the expression of genes involved in apoptosis (Bax, Bad, Bak1, p53, Bcl-2, Bcl-XL, Apaf-1, Caspase2, Caspase3a, Caspase9, and Caspase12) by quantitative real-time polymerase chain reaction.

Results: The IC50 value of styrax liquidus was found to be 125 μg/mL for 48 hours. According to the results, styrax liquidus reduced the population of HEp-2 laryngeal cancer cells and increased the expression of genes which were apoptosis related. These results indicate that styrax liquidus can be thought as a choice of cancer therapy.

Conclusion: The finding of the study showed that it would be more useful to perform more qualified studies about the effect of styrax liquidus on cancer cells.

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来源期刊
Eurasian Journal of Medicine
Eurasian Journal of Medicine Medicine-Medicine (all)
CiteScore
1.90
自引率
6.70%
发文量
59
审稿时长
16 weeks
期刊介绍: Eurasian Journal of Medicine (Eurasian J Med) is an international, scientific, open access periodical published by independent, unbiased, and triple-blinded peer-review principles. The journal is the official publication of Atatürk University School of Medicine and published triannually in February, June, and October. The publication language of the journal is English. The aim of the Eurasian Journal of Medicine is to publish original research papers of the highest scientific and clinical value in all medical fields. The Eurasian J Med also includes reviews, editorial short notes and letters to the editor that either as a comment related to recently published articles in our journal or as a case report. The target audience of the journal includes researchers, physicians and healthcare professionals who are interested or working in in all medical disciplines.
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