在接近阴道液的条件下,詹森尼乳杆菌菌株62B产生一种细菌素样抑制物质,靶向并消除加德纳菌。

IF 4.6 Q2 MATERIALS SCIENCE, BIOMATERIALS
Stephany Navarro, Habib Abla, Jane A Colmer-Hamood, Gary Ventolini, Abdul N Hamood
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引用次数: 0

摘要

在阴道生态系统中,乳酸杆菌和加德纳菌可能相互作用并影响彼此的生长,但这种相互作用的细节尚不清楚。使用模拟阴道液的培养基和两室共培养系统来防止细菌之间的细胞间接触,我们检测了詹森尼乳杆菌62B(Lj 62B)和/或皮奥蒂乳杆菌(Gp)JCP8151B产生细胞外因子的可能性,它们通过细胞外因子影响彼此的生存能力。到24 接种后h(hpi)在共培养系统中,在类似于阴道环境的条件下(pH 5.0,37) °C和5%CO2条件下,Lj 62B的活力没有受到影响,但Gp JCP8151B已被消除。从Lj 62B培养物(Lj CFS)在20hpi而不是16hpi收获的无细胞上清液也消除了Gp JCP8151B的生长。Lj62B产生的乳酸和H2O2都不是这种作用的原因。Lj-CFS不影响三种乳酸杆菌或八种革兰氏阳性和革兰氏阴性尿路病原体的生存能力,但消除了八种不同Gardnerella菌株的生存能力。蛋白酶处理消除了Lj-CFS中抑制因子的活性,热处理降低了其活性,这表明它很可能是细菌素样蛋白;分馏表明该因子的分子量在10-30之间 kDa范围。这些结果表明,在模拟阴道液和类似于阴道环境的生长条件的培养基中,Lj 62B产生一种潜在的细菌素样抑制物质(Lj BLIS),该物质明确靶向Gardnerella spp.菌株。一旦完全表征,Lj BLIS可能是一种潜在的治疗Gardnerella相关BV的方法,不会改变阴道菌群。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Under conditions closely mimicking vaginal fluid, Lactobacillus jensenii strain 62B produces a bacteriocin-like inhibitory substance that targets and eliminates Gardnerella species.

Within the vaginal ecosystem, lactobacilli and Gardnerella spp. likely interact and influence each other's growth, yet the details of this interaction are not clearly defined. Using medium simulating vaginal fluid and a two-chamber co-culturing system to prevent cell-to-cell contact between the bacteria, we examined the possibility that Lactobacillus jensenii 62B (Lj 62B) and/or G. piotii (Gp) JCP8151B produce extracellular factors through which they influence each other's viability. By 24 h post-inoculation (hpi) in the co-culture system and under conditions similar to the vaginal environment - pH 5.0, 37 °C, and 5% CO2, Lj 62B viability was not affected but Gp JCP8151B had been eliminated. Cell-free supernatant harvested from Lj 62B cultures (Lj-CFS) at 20 hpi, but not 16 hpi, also eliminated Gp JCP8151B growth. Neither lactic acid nor H2O2 production by Lj 62B was responsible for this effect. The Lj-CFS did not affect viability of three species of lactobacilli or eight species of Gram-positive and Gram-negative uropathogens but eliminated viability of eight different strains of Gardnerella spp. Activity of the inhibitory factor within Lj-CFS was abolished by protease treatment and reduced by heat treatment suggesting it is most likely a bacteriocin-like protein; fractionation revealed that the factor has a molecular weight within the 10-30 kDa range. These results suggest that, in medium mimicking vaginal fluid and growth conditions similar to the vaginal environment, Lj 62B produces a potential bacteriocin-like inhibitory substance (Lj-BLIS) that clearly targets Gardnerella spp. strains. Once fully characterized, Lj-BLIS may be a potential treatment for Gardnerella-related BV that does not alter the vaginal microflora.

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来源期刊
ACS Applied Bio Materials
ACS Applied Bio Materials Chemistry-Chemistry (all)
CiteScore
9.40
自引率
2.10%
发文量
464
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