<i>In vitro</i> Characterization of the Essential Oil Extracted from <i>Lavandula dentata</i> and its Application in the Field of Biotherapy.

&lt;b&gt;Background and Objective:&lt;/b&gt; &lt;i&gt;Lavandula dentata &lt;/i&gt;essential oil holds promise as a valuable natural resource with diverse therapeutic potential. The main objective of this study was to investigate the bioactivities of &lt;i&gt;Lavandula dentata &lt;/i&gt;essential oil, specifically its antimicrobial, analgesic, anti-diabetic and anticoagulant properties. &lt;b&gt;Materials and Methods:&lt;/b&gt; The Algerian medicinal plant &lt;i&gt;Lavandula dentata &lt;/i&gt;EO was collected from Tipaza City (Algeria). The hydro-distillation method was used to get yield of essential oil. The GC/MS analysis was done to identify the bioactive compound of &lt;i&gt;Lavandula dentata&lt;/i&gt; EO. The antimicrobial activity of&lt;i&gt; L. dentata &lt;/i&gt;essential oil (EO) was assessed using the disc diffusion method against eight different microorganisms. The antidiabetic and anticoagulant activity was also studied. &lt;b&gt;Results:&lt;/b&gt; The hydrodistillation method yielded approximately 0.4% of essential oil. The GC/MS results showed that &lt;i&gt;L. dentata&lt;/i&gt; EO contains a total of 18 elements and the main constituents were 1.8-cineole (41.48%) and β-pinene (33.43%). The EO exhibited a robust inhibitory effect on the growth of all tested microorganisms, with inhibitory diameters ranging from 16.6 to 38.5 mm. &lt;i&gt;Lavandula dentata&lt;/i&gt; EO presented anti-diabetic activity by inhibiting the α-amylase enzyme, with an IC&lt;sub&gt;50&lt;/sub&gt; value of approximately 135.08±0.25 μg mL&lt;sup&gt;–1&lt;/sup&gt;, demonstrating significant anti-diabetic activity and anti-coagulant activity. &lt;b&gt;Conclusion:&lt;/b&gt; &lt;i&gt;Lavandula dentata&lt;/i&gt; EO processes great antimicrobial, analgesic, anti- diabetic and anticoagulant properties. Further investigations into its mechanisms of action and safety profile are warranted to fully exploit its therapeutic potential.