人Fanconi贫血J组蛋白BLM结合区的表达、纯化及特性研究

IF 0.4 Q4 BIOCHEMICAL RESEARCH METHODS

Journal of the Korean magnetic resonance society Pub Date : 2016-03-20 DOI:10.6564/JKMRS.2016.20.1.022

Kyu-Hyeon Yeom, Chin-Ju Park

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引用次数: 0

摘要

【FANCJ】是一种DNA解旋酶，通过从5′到3′方向分解g -四重体DNA，有助于基因组的稳定性。除了主要的atp酶解旋酶核心外，FANCJ在其c端部分有蛋白结合区。BRCA1和BLM是FANCJ的结合伙伴，这些蛋白-蛋白相互作用有助于基因组的稳定性和对复制胁迫的适当反应。作为研究FANCJ-BLM相互作用的首次尝试，我们制备了FANCJ的BLM结合区，并用CD和NMR对其进行了表征。FANCJ(881-941)为N-ter 6xHis的低聚物。基于CD数据的二次结构预测表明，FANCJ(881-941)由${\beta}$片、匝和线圈组成。$^1H-^{15}N$ HSQC光谱显示峰强度不均匀，峰数少于结构中氨基酸的数量。结果表明，优化是进一步深入研究的必要条件。

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Expression, Purification and Characterization of the BLM binding region of human Fanconi Anemia Group J Protein

【FANCJ is a DNA helicase which contributes genome stability by resolving G-quadruplex DNA from 5' to 3' direction. In addition to main ATPase helicase core, FANCJ has the protein binding region at its C-terminal part. BRCA1 and BLM are the binding partner of FANCJ and these protein-protein interactions contribute genomic stability and the proper response to replication stress. As the first attempt for studying FANCJ-BLM interaction, we prepared BLM binding region of FANCJ and characterized with CD and NMR spectroscopy. FANCJ (881-941) with N-ter 6xHis was purified as the oligomer. Secondary structure prediction based on CD data revealed that FANCJ (881-941) composed with ${\beta}$ sheet, turn and coils. $^1H-^{15}N$ HSQC spectra showed nonhomogeneous peak intensities with less number of peaks comparing than the number of amino acids in the construct. It indicated that optimization should be necessary for detailed further structural studies.】

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Journal of the Korean magnetic resonance society BIOCHEMICAL RESEARCH METHODS-

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66.70%

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