不同种植材料培养的大鼠成骨细胞中钙、磷酸盐和碱性磷酸酶活性的体外水平

Q4 Medicine

Trends in Biomaterials and Artificial Organs Pub Date : 2013-05-27 DOI:10.7392/OPENACCESS.70081966

F. Shaama

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引用次数: 0

摘要

从新生儿Sprague-Dawley大鼠的颅骨中获取成骨细胞。在37℃，5% co2的潮湿气氛中，在钴铬(CO - cr)， cp钛(Ti)，聚甲基丙烯酸甲酯(PMMA)和木材存在的人工培养基中培养细胞。在两周内监测细胞功能。结果表明，材料对ca2 +和po4.2 -的影响不显著，但时间和相互作用显著(p<0.05)。对于ALP，材料、时间和相互作用显著(p<0.05)。木材产生的ALP活性最高。电子显微镜研究捕获了在所有材料上生长的细胞，仅在木材样品中存在ca2 +。

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In Vitro Levels of Calcium, Phosphate and Alkaline Phosphatase Activity in Media of Rat Osteoblast Grown in the Presence of Various Implant Materials

Osteoblast cells from newborn Sprague-Dawley rats were harvested from calvaria. Cells were cultivated in the presence of Cobalt-Chrome (Co-Cr), cpTitanium (Ti), polymethylmethacrylate (PMMA), and wood in artificial media in humidified atmosphere at 37 0 C, 5% CO 2 . Cellular function was monitored over two weeks. Results indicated materials were not significant for Ca 2+ , and PO 4 2- but the time and interaction was significant (p<0.05). For ALP, materials, time and interaction was significant (p<0.05). Wood produced the highest activity for ALP. Electron microscopic studies captured cells growing over all materials and the presence of Ca 2+ in wood samples only.

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来源期刊

Trends in Biomaterials and Artificial Organs Medicine-Medicine (miscellaneous)

CiteScore

0.70

自引率

0.00%

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