Eun-ji Lee, Hye-Sung Park, Chan-Jung Lee, W. Kong, C. Koo
{"title":"银耳菌丝体培养的适宜条件","authors":"Eun-ji Lee, Hye-Sung Park, Chan-Jung Lee, W. Kong, C. Koo","doi":"10.4489/KJM.20190001","DOIUrl":null,"url":null,"abstract":"This study was conducted to investigate the optimum culture conditions, for mass production of Tremella fuciformis in M9 basic medium. The strain KMCC04674, used in this study was identified T. fuciformis by internal transcribed spacer (ITS). To define the optimum conditions for the mass production of T. fuciformis, we investigated the effects of different culture conditions and various nutrient sources on the fungal growth. The optimum initial pH and temperature for the fungal growth were 5.0 and 25°C, respectively. The optimal composition of the growth medium was 4.0% mannitol, 3.0% NH4H2PO4, 1.0% malt extract, 1.0% glutamic acid, 5mM CaCl2, and 0.5% glucanic acid.","PeriodicalId":52172,"journal":{"name":"Korean Journal of Mycology","volume":"47 1","pages":"1-12"},"PeriodicalIF":0.0000,"publicationDate":"2019-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"1","resultStr":"{\"title\":\"Suitable Conditions for Mycelial Culture of Tremella fuciformis\",\"authors\":\"Eun-ji Lee, Hye-Sung Park, Chan-Jung Lee, W. Kong, C. Koo\",\"doi\":\"10.4489/KJM.20190001\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"This study was conducted to investigate the optimum culture conditions, for mass production of Tremella fuciformis in M9 basic medium. The strain KMCC04674, used in this study was identified T. fuciformis by internal transcribed spacer (ITS). To define the optimum conditions for the mass production of T. fuciformis, we investigated the effects of different culture conditions and various nutrient sources on the fungal growth. The optimum initial pH and temperature for the fungal growth were 5.0 and 25°C, respectively. The optimal composition of the growth medium was 4.0% mannitol, 3.0% NH4H2PO4, 1.0% malt extract, 1.0% glutamic acid, 5mM CaCl2, and 0.5% glucanic acid.\",\"PeriodicalId\":52172,\"journal\":{\"name\":\"Korean Journal of Mycology\",\"volume\":\"47 1\",\"pages\":\"1-12\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2019-01-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"1\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Korean Journal of Mycology\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.4489/KJM.20190001\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q4\",\"JCRName\":\"Environmental Science\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Korean Journal of Mycology","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.4489/KJM.20190001","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"Environmental Science","Score":null,"Total":0}
Suitable Conditions for Mycelial Culture of Tremella fuciformis
This study was conducted to investigate the optimum culture conditions, for mass production of Tremella fuciformis in M9 basic medium. The strain KMCC04674, used in this study was identified T. fuciformis by internal transcribed spacer (ITS). To define the optimum conditions for the mass production of T. fuciformis, we investigated the effects of different culture conditions and various nutrient sources on the fungal growth. The optimum initial pH and temperature for the fungal growth were 5.0 and 25°C, respectively. The optimal composition of the growth medium was 4.0% mannitol, 3.0% NH4H2PO4, 1.0% malt extract, 1.0% glutamic acid, 5mM CaCl2, and 0.5% glucanic acid.