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引用次数: 2
摘要
在本文中,我们非常简要地回顾了基于光学和电检测原理的DNA生物传感器,主要参考了我们过去应用这两种技术的工作,但这里使用几乎相同的传感器芯片表面结构,即基于脉冲等离子体沉积方案制备的捕获探针层,用于马来酸酐和随后的湿化学附着胺功能化肽核酸(PNA)探针寡核苷酸。在5 '端贴上cy5色团标记的15 mer DNA靶链用于表面等离子体光学检测,而未标记的相同靶DNA用于基于OTFT传感器的检测,其中结合(杂交)DNA分子的单纯电荷密度调节源漏电流。详细介绍了该装置的传感机理和检测限。这两种技术都可以监测表面杂交反应,并提供定量区分具有不同程度不匹配序列的目标的能力。
In this paper, we very briefly review DNA biosensors based on optical and electrical detection principles, referring mainly to our past work applying both techniques but here using nearly identical sensor chip surface architectures, i.e., capture probe layers that were prepared based on a pulsed plasma deposition protocol for maleic anhydride and subsequent wet-chemical attachment of the amine-functionalized peptide nucleic acid (PNA) probe oligonucleotides. 15 mer DNA target strands, labeled with Cy5-chromophores that were attached at the 5’ end were used for surface plasmon optical detection and the same target DNA but without label was used in OTFT sensor-based detection where the mere charge density of the bound (hybridized) DNA molecules modulate the source-drain current. The sensing mechanisms and the detection limits of the devices are described in some detail. Both techniques allow for the monitoring of surface hybridization reactions, and offer the capacity to quantitatively discriminate between targets with different degrees of mismatched sequences.