{"title":"利用issr标记鉴定非洲热蝇的性别","authors":"L. Iglesias-Andreu, Enrique Favián-Vega","doi":"10.47743/jpd.2021.28.1.897","DOIUrl":null,"url":null,"abstract":"Zamia inermis is a dioecious cycad endemic to the State of Veracruz that is on the risk extinction. Sexspecific markers are important in understanding the mechanism of sex determination. However, since little is known about the mechanism of sex determination in Z. inermis, we proposed looking for Inter-simple Sequence Repeats (ISSR) markers that could be linked to sexual expression in this species. Using DNA bulk samples of male and female genotypes and 6 ISSR primers, a female marker (~867 bp) was identified with primer ISSR-18, which was present in 66% of the DNA mixtures of the female genotypes analyzed. The results of the Principal Coordinate Analysis performed revealed a tendency for clustering of genotypes of the same sex.","PeriodicalId":16754,"journal":{"name":"Journal of Plant Development","volume":"1 1","pages":""},"PeriodicalIF":0.0000,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"IDENTIFICATION OF SEX IN ZAMIA INERMIS USING ISSR MARKERS\",\"authors\":\"L. Iglesias-Andreu, Enrique Favián-Vega\",\"doi\":\"10.47743/jpd.2021.28.1.897\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Zamia inermis is a dioecious cycad endemic to the State of Veracruz that is on the risk extinction. Sexspecific markers are important in understanding the mechanism of sex determination. However, since little is known about the mechanism of sex determination in Z. inermis, we proposed looking for Inter-simple Sequence Repeats (ISSR) markers that could be linked to sexual expression in this species. Using DNA bulk samples of male and female genotypes and 6 ISSR primers, a female marker (~867 bp) was identified with primer ISSR-18, which was present in 66% of the DNA mixtures of the female genotypes analyzed. The results of the Principal Coordinate Analysis performed revealed a tendency for clustering of genotypes of the same sex.\",\"PeriodicalId\":16754,\"journal\":{\"name\":\"Journal of Plant Development\",\"volume\":\"1 1\",\"pages\":\"\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2021-01-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of Plant Development\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.47743/jpd.2021.28.1.897\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Plant Development","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.47743/jpd.2021.28.1.897","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
IDENTIFICATION OF SEX IN ZAMIA INERMIS USING ISSR MARKERS
Zamia inermis is a dioecious cycad endemic to the State of Veracruz that is on the risk extinction. Sexspecific markers are important in understanding the mechanism of sex determination. However, since little is known about the mechanism of sex determination in Z. inermis, we proposed looking for Inter-simple Sequence Repeats (ISSR) markers that could be linked to sexual expression in this species. Using DNA bulk samples of male and female genotypes and 6 ISSR primers, a female marker (~867 bp) was identified with primer ISSR-18, which was present in 66% of the DNA mixtures of the female genotypes analyzed. The results of the Principal Coordinate Analysis performed revealed a tendency for clustering of genotypes of the same sex.
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