P35 Plastination: Experiences with delayed impregnation

A.H. Weiglein Institute of Anatomy Medical University of Graz Graz, Austria ABSTRACT: During an educational demonstration of the P35 technique, brain slices which had been immersed in P35 resin and stored in a cold room (5° Celsius) for approximately two years were used. The resin was very viscous and it was difficult to remove the steel basket containing the brain slices from the container of resin. There were technical difficulties during the manipulation of the slices: slices were brittle and fragile, filter paper spacers were stuck to the specimens, curing had begun where the slice touched the grid and gel-like resin remnants were stuck on the metal grids. Despite the very long immersion period and the problems encountered, the final specimen was satisfactory from an optical point of view.