尼日利亚东南部Nnewi使用p57kip2免疫染色对葡萄胎形态诊断的流行病学方面和诊断准确性-一项多中心研究

Q3 Health Professions
C. Ndukwe, C. Ukah
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引用次数: 0

摘要

尼日利亚关于葡萄胎(HM)的研究很多,但缺乏组织学诊断,也没有区分完全葡萄胎(CHM)和部分葡萄胎(PHM)。此外,这些研究均未涉及免疫组化(IHC)诊断或评估形态学诊断CHM的准确性。本研究的目的是利用p57KIP2免疫组化染色确定CHM的流行病学和形态学诊断的准确性。材料和方法:从档案中检索尼日利亚东南部Nnewi的两个组织病理学实验室中组织学诊断的CHM和PHM病例的石蜡块。对p57KIP2进行免疫组化染色。结果:在本研究中,我们回顾并重新分类了54例常规染色的HMs,其中15例(27.8%)为CHMs, 39例(72.2%)为PHMs。然而,在IHC染色后,我们进一步将其重新分类为21例(38.9%)CHMs和33例(61.1%)PHMs。常规苏木精和伊红(H和E)染色与免疫组化p57KIP2染色的组织病理诊断不一致8例(占研究病例的14.8%):1例为假阳性诊断,其余7例为假阴性诊断。由此可见,H和E对组织病理学诊断CHM病例的敏感性和特异性分别为66.7%和97%,阳性预测值为93.3%,阴性预测值为82.1%,总准确率为85.2%。结论:单纯形态学检查不能准确诊断CHM。因此,我们强烈建议在所有疑似HM病例中常规使用p57KIP2免疫染色。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Epidemiological aspects and diagnostic accuracy of morphological diagnosis of hydatidiform mole using p57kip2 immunostain in Nnewi, South-East Nigeria – A multicenter study
Introduction: There have been numerous studies on hydatidiform mole (HM) in Nigeria, but some lacked histological diagnosis, and others did not differentiate complete hydatidiform mole (CHM) from partial hydatidiform mole (PHM). In addition, none of these studies involved immunohistochemical (IHC) diagnosis or assessed the accuracy of morphologic diagnosis of CHM. The objective of this study is to determine the epidemiology and accuracy of morphologic diagnosis of CHM using p57KIP2 IHC stain. Materials and Methods: The paraffin blocks of histologically diagnosed cases of CHM and PHM seen at two histopathology laboratories in Nnewi, South-East Nigeria, were retrieved from the archives. IHC staining for p57KIP2 was done. Results: In this study, we reviewed and reclassified 54 cases of routinely stained HMs as 15 (27.8%) CHMs and 39 (72.2%) PHMs. However, following IHC staining, we further reclassified them as 21 (38.9%) CHMs and 33 (61.1%) PHMs. Discordant histopathological diagnosis between routine staining with hematoxylin and eosin (H and E) and IHC staining with p57KIP2 was identified in eight cases (which constitutes 14.8% of the studied cases): one case was a false-positive diagnosis, while the remaining seven cases were false-negative diagnosis. Thus, the sensitivity and specificity of histopathological diagnosed cases of CHM by H and E were 66.7% and 97%, respectively, with a positive predictive value of 93.3%, negative predictive value of 82.1%, and total accuracy of 85.2%. Conclusions: Morphology alone is suboptimal for accurate diagnosis of CHM. We therefore strongly recommend the routine use of p57KIP2 immunostain in all suspected cases of HM.
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来源期刊
Journal of Nature and Science of Medicine
Journal of Nature and Science of Medicine Health Professions-Health Professions (miscellaneous)
CiteScore
1.10
自引率
0.00%
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0
审稿时长
31 weeks
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