计算图像扫描密度法测定药物制剂中双氯芬酸钠的含量

IF 0.6 4区化学 Q4 CHEMISTRY, MULTIDISCIPLINARY

Journal of the chemical society of pakistan Pub Date : 2023-01-01 DOI:10.52568/001248/jcsp/45.03.2023

Waheed uz Zuman Waheed uz Zuman, Rida Ayub Rida Ayub, Amara Dar and Jamil Anwar Amara Dar and Jamil Anwar

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引用次数: 0

摘要

双氯芬酸钠是一种非甾体抗炎药，通过减少体内引起疼痛和炎症的物质来治疗。如果服药过量，总是有心脏病发作和中风的危险。本研究设计了一种简便、快速、经济的方法，用于测定当地药品制剂中双氯芬酸钠的含量。该方法基于药物与2,4 -二硝基苯基肼(2,4 -二硝基苯基肼)的反应。微量药物与2,4- dnph混合在预涂TLC板上，在碘酸钾和氢氧化锂的存在下，呈现绿色斑点。利用平板扫描仪对光斑进行扫描，并借助定制的软件对图像进行计算量化，测量光密度。优化了反应参数，并与标准分光光度法进行了比较

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Determination of Diclofenac Sodium in Pharmaceutical Preparations by Computational Image Scanning Densitometry

Diclofenac sodium is a nonsteroidal anti-inflammatory drug which cures by reducing substances in the body that cause pain and inflammation. There is always a risk of heart attack and stroke in case of taking excess of the drug. In present study a simple, fast and cost-effective method is devised for the assay of diclofenac sodium in locally available pharmaceutical preparations. The method is based on the reaction of the drug with 2, 4-DNPH (2, 4-Dinitro phenyl hydrazine). Micro-quantities of the drug gave green coloured spots when mixed with 2,4-DNPH on a pre-coated TLC plate, in the presence of potassium iodate and lithium hydroxide. The spots were scanned by using a flatbed scanner and the images obtained were computationally quantified with the help of custom-made software to measure the optical density. The reaction parameters were optimized, and the results were compared with the standard spectrophotometric method

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来源期刊

Journal of the chemical society of pakistan 化学-化学综合

CiteScore

1.30

自引率

14.30%

发文量

审稿时长

3.4 months

期刊介绍： This journal covers different research areas in the field of Chemistry. These include; Analytical Chemistry, Applied Chemistry, Biochemistry, Environmental Chemistry, Industrial Chemistry, Inorganic Chemistry, Organic Chemistry and Physical Chemistry. The journal publishes full length articles and Reviews from researchers in academia in addition to featuring comments. Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry.