快速和简单的microRNA Northern印迹

Biochemistry Insights Pub Date : 2009-01-01 DOI:10.4137/BCI.S2257

Nham T Tran

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引用次数: 5

摘要

RNA northern blots提供了基因表达的可靠测量。本报告中描述的简单northern blot技术已经过优化，可快速、可重复地检测和分析成熟和前体形式的microrna。该协议节省了商用组件的使用，其次将杂交步骤减少到2小时。

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Fast and Simple microRNA Northern Blots

RNA northern blots provide robust measurements of gene expression. The simple northern blot technique described in this report has been optimised to provide rapid, reproducible detection and analysis of mature and precursor forms of microRNAs. This protocol economises on the use of commercially available components and secondly reduces the hybridisation step to 2 hours.

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来源期刊

Biochemistry Insights BIOCHEMISTRY & MOLECULAR BIOLOGY-

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