蜡样芽孢杆菌CA15产胞外蛋白酶的最佳条件研究

Q3 Agricultural and Biological Sciences
F. Uyar, Ilknur Porsuk, G. Kızıl, E. Yilmaz
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引用次数: 86

摘要

从土壤中分离到一株碱性蛋白酶产生菌芽孢杆菌CA15。通过16S核糖体DNA测序,发现该微生物与蜡样芽孢杆菌亲缘关系较近。从碳源、氮源、金属离子、pH和温度等方面对培养条件进行了优化。在培养基中添加1%脱脂牛奶、1%淀粉和0.6% mgso4.7 h2o,初始pH为8.0,温度为35℃时,蛋白酶产量最大。在细胞密度达到1.8 × 108个/mL时,酶产量最高。发现在无机氮源的存在下,蛋白酶的水平较低。在蔗糖和乳糖的存在下,蛋白酶的产量减少。杆菌CA15碱性蛋白酶对Triton X-100、Tween 20和SDS具有极强的稳定性。PMSF对酶活性的抑制表明在活性位点存在丝氨酸残基。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Optimal conditions for production of extracellular protease from newly isolated Bacillus cereus strain CA15
An alkaline protease producer Bacillus sp. strain CA15 was isolated from soil. The microorganism was found to be closely related to Bacillus cereus based on 16S ribosomal DNA sequencing. The culture conditions for higher protease production were optimized with respect to carbon and nitrogen sources, metal ions, pH and temperature. Maximum protease production was obtained in the medium supplemented with 1% skim milk, 1% starch and 0.6% MgSO 4 .7H 2 O, initial pH 8.0 at 35°C. The best enzyme production was obtained during the stationary phase in which the cell density reached to 1.8x108 cells/mL. The level of protease was found to be low in the presence of inorganic nitrogen sources. The protease production was diminished in the presence of sucrose and lactose. The extreme stability towards Triton X-100, Tween 20 and SDS was observed by Bacillus sp. CA15 alkaline protease. The enzyme activity was inhibited by PMSF suggested that presence of serine residues at the active sites.
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来源期刊
Eurasian Journal of Biosciences
Eurasian Journal of Biosciences Agricultural and Biological Sciences-Agricultural and Biological Sciences (all)
CiteScore
1.20
自引率
0.00%
发文量
0
期刊介绍: EurAsian Journal of BioSciences (Abbrev. Eurasia J Biosci or EJOBIOS) is an international, refereed electronic journal. It publishes the results of original research in the field of biological sciences restricted tomorphology, physiology, genetics, taxonomy, ecology and biogeography of both prokaryotic and eucaryotic organisms. The journal encourages submission of manuscripts dealing with plant biology, animal biology, plant physiology, microbiology, hydrobiology, ecology and environmental science, ethnobiology, biodiversity and conservation biology. EurAsian Journal of BioSciences publishes original articles in the following areas: -Agriculture, Fisheries & Food -Anatomy & Morphology -Behavioural Sciences -Biology, Biochemistry and Biotechnology -Biophysics -Biology Education -Cellular Biology and Anatomical Sciences -Ecology, Evolution & Environment -Entomology -Forestry -General Biology -Genetics & Heredity -Life Sciences - Other topics -Microbiology and Immunology -Molecular Biology -Mycology -Palaeontology -Parasitology -Pharmacology & Pharmacy -Physiology and Related Sciences -Plant Sciences -Toxicology -Veterinary Sciences -Virology -Zoology
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