利用RAPD、ITS-PCR和RFLP分析花生茎腐病菌核菌的分子变异

Q3 Agricultural and Biological Sciences

Eurasian Journal of Biosciences Pub Date : 2010-07-01 DOI:10.5053/EJOBIOS.2010.4.0.10

S. D. Prasad, S. T. Basha, N. P. E. Reddy

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引用次数: 16

摘要

采用RAPD、ITS-PCR和RFLP等分子技术对罗氏菌核菌毒力分离株的遗传变异进行了研究。RAPD条带模式通过形成两个簇反映了分离物间的遗传多样性。5条RAPD引物共生成了221条可重复且可评分的多态性条带，长度从100 bp到2500 bp不等。用ITS1和ITS4通用引物扩增ITS区rDNA，扩增结果约为650 ~ 700 bp，证实所有分离株均为罗尔夫菌核菌。ITS-RFLP研究表明，使用限制性内切酶的分离株的限制性带型不存在多态性。

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Molecular variability among the isolates of Sclerotium rolfsii causing stem rot of groundnut by RAPD, ITS-PCR and RFLP

Genetic variability among the virulent isolates of Sclerotium rolfsii was studied using molecular techniques like RAPD, ITS-PCR and RFLP. The RAPD banding pattern reflected the genetic diversity among the isolates by formation of two clusters. A total of 221 reproducible and scorable polymorphic bands ranging approximately as low as 100 bp to as high as 2500 bp were generated with five RAPD primers. ITS region of rDNA amplification with specific ITS1 and ITS4 universal primers produced approximately 650 to 700 bp in all the isolates confirmed that all the isolates obtained are Sclerotium rolfsii. Studies by ITS-RFLP indicated that there was no polymorphism in restriction banding pattern among the isolates with the restriction endonucleases used.

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来源期刊

Eurasian Journal of Biosciences Agricultural and Biological Sciences-Agricultural and Biological Sciences (all)

CiteScore

1.20

自引率

0.00%

发文量

期刊介绍： EurAsian Journal of BioSciences (Abbrev. Eurasia J Biosci or EJOBIOS) is an international, refereed electronic journal. It publishes the results of original research in the field of biological sciences restricted tomorphology, physiology, genetics, taxonomy, ecology and biogeography of both prokaryotic and eucaryotic organisms. The journal encourages submission of manuscripts dealing with plant biology, animal biology, plant physiology, microbiology, hydrobiology, ecology and environmental science, ethnobiology, biodiversity and conservation biology. EurAsian Journal of BioSciences publishes original articles in the following areas: -Agriculture, Fisheries & Food -Anatomy & Morphology -Behavioural Sciences -Biology, Biochemistry and Biotechnology -Biophysics -Biology Education -Cellular Biology and Anatomical Sciences -Ecology, Evolution & Environment -Entomology -Forestry -General Biology -Genetics & Heredity -Life Sciences - Other topics -Microbiology and Immunology -Molecular Biology -Mycology -Palaeontology -Parasitology -Pharmacology & Pharmacy -Physiology and Related Sciences -Plant Sciences -Toxicology -Veterinary Sciences -Virology -Zoology