埃塞俄比亚牛呼吸道疾病主要病原菌的毒力基因鉴定和血清分型

微生物学(英文) Pub Date : 2022-01-01 DOI:10.4236/aim.2022.121002

Mirtneh Akalu, T. Abayneh, E. Gelaye, Getaw Derese, Behailu Tefera, T. Degefa, Vemulapati Bhadra Murthy

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引用次数: 0

摘要

牛呼吸道疾病(BRD)在所有年龄的牛中引起一种严重的肺炎。本研究旨在调查埃塞俄比亚BRD暴发样本中主要细菌病原体的荚膜型、血清型和毒力相关基因的分布。本研究共收集临床病人(107例)和肺炎肺组织(59例)166份样本。实验室鉴定分离出溶血支原体37(22.29%)、多杀假体25(15.06%)、海藻藻支原体12(7.23%)和嗜睡支原体15(9.04%)。多杀假单胞菌荚膜分型结果显示，A帽(hyaD-hyaC) 21株(84.0%)，D帽(dcbF) 4株(16.0%)。从26株(70.27%)、4株(10.81%)和7株(18.92%)分离得到溶血分枝杆菌血清型分别为A: 1、A: 2和A: 6。多杀假单胞菌生物分型结果显示，从3株中分离出A: 1、A: 2、A: 3株(14.29%)、2株(9.52%);

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Virulence Gene Characterization and Serotyping of Major Bacterial Pathogens Isolated from Bovine Respiratory Disease in Ethiopia

Bovine Respiratory Disease (BRD) causes a severe form of pneumonia in all age of cattle. This study was designed to investigate the distribution of capsular types, serotypes, and virulence-associated genes of the major bacterial pathogens from BRD outbreak samples in Ethiopia. In this study 166 samples were collected from clinically sick (n = 107) and pneumonic lung tissue (n = 59). Laboratory assay confirmed isolation of M. haemolytica 37 (22.29%), P. multocida 25 (15.06%), B. trehalosi 12 (7.23%), and H. somni 15 (9.04%). PCR assay of P. multocida capsular typing revealed 21 (84.0%) cap A (hyaD-hyaC) and 4 (16.0%) cap D (dcbF) strains. M. haemolytica serotypes belonged to A: 1, A: 2, and A: 6 from 26 (70.27%), 4 (10.81%), and 7 (18.92%) isolates, respectively. P. multocida biotyping showed isolation of A: 1, A: 2, and A: 3 from 3 (14.29%), 2 (9.52%),

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来源期刊

微生物学(英文)

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661