小环状RNA在原核翻译系统中的滚动圈扩增

IF 16.1 1区化学 Q1 CHEMISTRY, MULTIDISCIPLINARY

Angewandte Chemie International Edition Pub Date : 2013-05-28 DOI:10.1002/anie.201302044

Naoko Abe, Dr. Michio Hiroshima, Hideto Maruyama, Dr. Yuko Nakashima, Dr. Yukiko Nakano, Prof.?Dr. Akira Matsuda, Prof.?Dr. Yasushi Sako, Prof.?Dr. Yoshihiro Ito, Prof.?Dr. Hiroshi Abe

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引用次数: 73

摘要

绕过:含有无限开放阅读框的小环状RNA分子被合成并在大肠杆菌无细胞翻译系统中进行测试。研究发现，长度为126个核苷酸的环状RNA比线性RNA产生的产物多两个数量级，这是因为在这种连续的肽合成中，核糖体对环状RNA的延伸比对线性RNA的延伸更有效。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

Rolling Circle Amplification in a Prokaryotic Translation System Using Small Circular RNA†

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Rolling Circle Amplification in a Prokaryotic Translation System Using Small Circular RNA†

Getting the runaround: Small circular RNA molecules containing an infinite open reading frame were synthesized and tested in an E. coli cell-free translation system. A circular RNA 126 nucleotides in length was found to produce more product than its linear counterpart by two orders of magnitude, because a ribosome can work more effectively towards the elongation on circular RNA than it can on linear RNA in this continuous peptide synthesis.

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来源期刊

Angewandte Chemie International Edition 化学-化学综合

CiteScore

26.60

自引率

6.60%

发文量

3549

审稿时长

1.5 months

期刊介绍： Angewandte Chemie, a journal of the German Chemical Society (GDCh), maintains a leading position among scholarly journals in general chemistry with an impressive Impact Factor of 16.6 (2022 Journal Citation Reports, Clarivate, 2023). Published weekly in a reader-friendly format, it features new articles almost every day. Established in 1887, Angewandte Chemie is a prominent chemistry journal, offering a dynamic blend of Review-type articles, Highlights, Communications, and Research Articles on a weekly basis, making it unique in the field.