重组牛胰蛋白酶抑制剂对四氯化碳所致大鼠慢性肝损伤的保护作用

Q4 Pharmacology, Toxicology and Pharmaceutics

中国药理学与毒理学杂志 Pub Date : 2008-06-01 DOI:10.3867/J.ISSN.1000-3002.2008.03.007

L. Yang, J. He, Wen Dong, Xin Zhang, C. Zhou, X. Ren, Weiqun Yan

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引用次数: 0

摘要

目的观察重组牛胰蛋白酶抑制剂(rBPTI)对慢性肝损伤的保护作用。方法98只大鼠随机分为正常对照组、模型对照组、rBPTI 20、40、80 MU·kg-1组、抑肽蛋白80 MU·kg-1组和肝细胞生长促进因子(pHGF) 100 mg·kg-1组。采用四氯化碳(CCl4)诱导大鼠慢性肝损伤模型。8周后，除正常组和模型对照组外，每天给予rBPTI、抑蛋白素或pHGF (ip)，连续4周。测定大鼠血清中谷丙转氨酶(GPT)、谷草转氨酶(GOT)活性、白蛋白(Alb)含量、白蛋白与球蛋白含量比(A/G)、唾液酸(SA)含量、羟脯氨酸(Hyp)水平及肝组织病理变化。结果与正常对照组比较，模型组大鼠血清GPT、GOT活性升高，血清SA和肝组织Hyp含量升高，血清Alb含量和A/G比值降低。与模型组比较，rBPTI(20、40、80 MU·kg-1)可降低血清GPT、GOT活性，降低血清SA含量，降低肝组织Hyp含量。rBPTI可提高血清白蛋白含量和A/G比值。rBPTI对慢性肝损伤如脂肪变性、肝纤维化有明显的缓解作用。结论rBPTI对CCl4诱导的慢性肝损伤具有保护和抗纤维化作用，且在观察剂量下其作用与抑酶蛋白相当。

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Protective effect of recombinant bovine pancreatic trypsin inhibitor on chronic liver injury induced by carbon tetrachloride in rats

AIM To observe the protection of recombinant bovine pancreatic trypsin inhibitor(rBPTI) against chronic liver injury.METHODS Ninety eight rats were randomized into 7 groups:normal control,model control,rBPTI 20,40 and 80 MU·kg-1,aprotinin 80 MU·kg-1 and hepatocyte growth-promoting factor(pHGF) 100 mg·kg-1 groups.The chronic liver injury rat model was induced by carbon tetrachloride(CCl4).After 8 weeks,the rats were administered(ip) with rBPTI,aprotinin or pHGF daily for 4 weeks except that in normal and model control groups.The glutamic-pyruvic transaminase(GPT) and glutamic-oxalacetic transaminase(GOT) activities,albumin(Alb) content,Alb and globulin content ratio(A/G),sialic acid(SA) content in serum were measured.Hydroxyproline(Hyp) level and histopathological changes of hepatic tissue were also examined.RESULTS Compared with normal control group,the activities of serum GPT and GOT,and the contents of SA in serum and Hyp in liver tissue in model group were increased,and the content of serum Alb and A/G ratio were decreased.Compared with model group,rBPTI(20,40 and 80 MU·kg-1) decreased the activities of GPT and GOT,and the contents of SA in serum and Hyp in liver tissue.The content of serum Alb and A/G ratio were increased by rBPTI.In addition,rBPTI alleviated chronic hepatic injuries such as fatty degeneration and liver fibrosis significantly.CONCLUSION rBPTI has protective and anti-fibrosis effects against chronic liver injury induced by CCl4,and the efficiency is equivalent as aprotinin in the observed dosages.

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来源期刊

中国药理学与毒理学杂志 Pharmacology, Toxicology and Pharmaceutics-Pharmacology

CiteScore

0.20

自引率

0.00%

发文量

6787

期刊介绍： Chinese Journal of Pharmacology and Toxicology was first published in November 1986. As a journal of high academic value jointly edited by the Chinese Pharmacological Society, Chinese Society of Toxicology and the Academy of Military Medical Sciences (AMMS), it is a dominating one in China’s biomedicine, devoted to publication of research findings, reviews of special topic and new technologies and methods in every branch of experimental toxicology and pharmacology.