菲律宾红树林芽孢杆菌产纤维素酶的筛选与优化

Q3 Environmental Science

Philippine Journal of Systematic Biology Pub Date : 2010-06-11 DOI:10.3860/PJSB.V4I0.1566

Nik Shawn C. Tabao, R. Monsalud

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引用次数: 15

摘要

先前从菲律宾几个红树林地区分离的225株红树林细菌菌株进行了纤维素酶生产的定性筛选，发现154株能够生产纤维素酶。其中10株纤维素酶活性很强，并进行了定量分析。5个活性最高的菌株BBCS-11、BBCS-14、BBoB2L2-2、BOrMGS-2和BOrMGS-3，包括Cellulomonas sp. BIOTECH 1240(对照)，进一步进行了部分优化研究。考察了底物、pH、培养温度和培养时间对纤维素酶产率的影响。优化条件下菌株的纤维素酶活性(U mL -1)为:54.80(对照);56.60 (BBCS-11);66.50 (BBCS-14);50.33 (BBoB2L2-2);51.04 (BOrMGS-2);48.70 (BOrMGS-3)。有前途的纤维素酶产生菌已被先前确定:BBCS-11为蜡样芽孢杆菌;BBCS-14为地衣芽孢杆菌;BOrMGS-2和BOrMGS-3为矮芽孢杆菌;BBoB2L2-2为芽孢杆菌。

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SCREENING AND OPTIMIZATION OF CELLULASE PRODUCTION OF BACILLUS STRAINS ISOLATED FROM PHILIPPINE MANGROVES

Two-hundred and twenty-five mangrove bacterial strains previously isolated from several mangrove areas in the Philippines were qualitatively screened for cellulase production and 154 were found to be capable of cellulase production. Among them, ten strains showed very strong positive cellulase activities, which were then quantified. Five with the statistically highest activities, specifically, BBCS-11, BBCS-14, BBoB2L2-2, BOrMGS-2, and BOrMGS-3, including Cellulomonas sp. BIOTECH 1240 (control), were further subjected to partial optimization studies. The effects of substrate, pH, incubation temperature and incubation time on cellulase productoin were determined. The cellulase activities (U mL -1 ) of the strains under optimized conditions were: 54.80 (Control); 56.60 (BBCS-11); 66.50 (BBCS-14); 50.33 (BBoB2L2-2); 51.04 (BOrMGS-2); and 48.70 (BOrMGS-3). The promising cellulase producers were previously identified: BBCS-11 as Bacillus cereus; BBCS-14 as Bacillus licheniformis; BOrMGS-2 and BOrMGS-3 as Bacillus pumilus; and BBoB2L2-2 as Bacillus sp.

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来源期刊

Philippine Journal of Systematic Biology Environmental Science-Ecology

CiteScore

1.50

自引率

0.00%

发文量

期刊介绍： The Philippine Journal of Systematic Biology (Print ISSN: 1908-6865; Online ISSN: 2508-0342) is an annual, peer-reviewed journal in English that publishes high quality reports of original research and reviews in the field of Taxonomy & Systematics, Ecology and Conservation Biology. The PJSB is a CHED recognized journal under CHED CMO 50 series of 2017. It is also included in the Clavariate Analysis (formerly a subsidiary of Thomson Reuters) Master Journal List under Zoological Record, BIOSIS Previews and Biological Abstracts.The PJSB is the official publication of the Association of Systematic Biologists of the Philippines (SEC Registration: Association of Philippine Taxonomists, Inc.). The organization financially supports the journal and all its endeavors. The journal aims to build up quality information on animal, plant and microbial diversity in the Philippines. Articles dealing with original research or reviews in Zoological, Botanical or Microbial Systematics, Ecology and Conservation Biology are welcome. Authors are advised to consult a recent issue of PJSB for the current format and style. All manuscripts are reviewed by at least two qualified referees.