极危蝾螈15颗多态微卫星的发育与特性研究

IF 1.2 3区生物学 Q2 ZOOLOGY

Asian Herpetological Research Pub Date : 2014-01-01 DOI:10.3724/SP.J.1245.2014.00281

Weizhao Yang, Jie Wang, Jianping Jiang, Cheng Li, F. Xie

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引用次数: 0

摘要

中国海棘螈(Echinotriton chinhaiensis)是一种极危蝾螈，其分布仅限于中国浙江省宁波市。在本研究中，我们从二核苷酸和四核苷酸富集文库中开发并鉴定了15个多态微卫星位点。等位基因数量在4 ~ 12个之间，平均每个位点有7.27个等位基因。观察杂合度和期望杂合度分别为0.250 ~ 0.844和0.511 ~ 0.872，平均值分别为0.596和0.722;分别。本文所描述的微卫星多态性位点为进一步研究中国海桑的遗传多样性和基因流提供了依据，有助于制定有效的保护策略。

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Development and Characterization of Fifteen Polymorphic Microsatellites for Extremely Endangered Salamander, Echinotriton chinhaiensis

Echinotriton chinhaiensis is a critically endangered salamander and its distribution is restricted to Ningbo, Zhejiang Province of China. In this study, we developed and characterized fifteen polymorphic microsatellite loci for E. chinhaiensis from dinucleotide- and tetranucleotide- enriched library. The number of alleles ranges from 4 to 12 with an average of 7.27 alleles per locus. The observed and expected heterozygosities values were from 0.250 to 0.844 and 0.511 to 0.872 with an average value of 0.596 and 0.722; respectively. The polymorphic microsatellite loci described in this paper are useful in the further study on genetic diversity and gene flow, which would be helpful to formulate effective conservation strategies for the E. chinhaiensis.

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来源期刊

Asian Herpetological Research ZOOLOGY-

CiteScore

2.80

自引率

7.10%

发文量

441

审稿时长

>12 weeks

期刊介绍： AHR aims to provide a forum for herpetologists and related scientists interested in conducting international academic exchanges and joint studies, and a platform for introducing their newly made scientific and technological data, and publishing their research results and achievements in the world, but focusing on the Asian-Pacific Region. The principal criteria of AHR for acceptance of articles for publication are the quality and significance of the research, breadth of interest of the work to the readership, and the clarity and effectiveness of communication. AHR welcomes submission of manuscripts from authors in all countries of the world, though with a focus on the herpetological studies in the Asian and Pacific Region, including major articles, shorter communications and review articles.