Prevalence, Diagnosis and Therapeutic Management of Subclinical Mastitis in Deoni Cows

The study was carried out to know prevalence, diagnosis and therapeutic efficacy of Marbofloxacin and Vitamin E and Selenium against the subclinical mastitis from lactating Deoni Cows at Organized and Unorganized dairy farms in and around Bidar. Screening of subclinical mastitis was done by California Mastitis Test (CMT), Electrical Conductivity (EC) and Somatic Cell Count (SCC) in milk. Positive samples for subclinical mastitis were considered for isolation and identification of bacterial pathogen by cultural examination. Ninty (56.25%) out of one hundred sixty lactating cows were found positive for subclinical mastitis. Affected quarter-wise single, two, three and four prevalence was given a prevalence of 28.13, 25, 1.88 per cent. Out of 21 positive milk sample 16 and 9 samples were found positive for Staphylococcus spp. Streptococcus spp. (76.19%) and E.coli (42.86%) organism. The antibiogram has a of the bacterial isolates to standard antibiotic discs determined by disc diffusion method revealed the highest sensitivity to Gentamicin and Enrofloxacin followed by Tetracycline, Moxifloxacin, Cefoperazone, Ceftriaxone, in decreasing order and least sensitive to amoxicillin and clavulanic acid. Positive animal were treated with single dose of Marbofloxacin (8 mg/kg.BW) alone, Marbofloxacin with Vitamin E and Selenium and Vitamin E and Selenium alone on ‘0’day and 3rd day with (1 ml/25 kg.BW) given intramuscularly. Therapeutic efficacy of Marbofloxacin along with Vitamin E and Selenium showed (86.67%), Marbofloxacin (84.62%) and Vitamin E and Selenium (50%).