Resveratrol Nanoemulsion: A Promising Protector Against Ethinylestradiol-Induced Hepatic Cholestasis in Female Rats

Ethinylestradiol (EE) induced cholestasis was reported in female rats-model. The present article aimed to investigate anticholestatic activity of Resveratrol Nanoemulsion (RENE) against EE-induced cholestasis in adult female rats. The mean particle size of RENE was 49.5 ± 0.05 nm and zeta potential of +15.75 with the observed shapes of nanoparticle was spherical. Also, the median lethal dose (LD50) of RENE in rats was 795 mg/kg body weight. Administration of 1/20 LD50 RENE (39.75 mg/kg.b.w.) to EE–treated rats normalize serum cholesterol level as well as against an increase of serum TBA, bilirubin concentration. The treatment also resulted in a significant increase in hepatic SOD and GPx. RENE inhibited serum ALP, ALT and γ-GT activities, as well as reduced serum TNF-α, NO, MMP-2 MMP-9 and hepatic MDA as compared to EE-treated rats. The results clearly suggest that RENE has a powerful prophylactic action in cholestasis induced by EE.